Immunosuppressive effects of sialostatin L1 and L2 isolated from the taiga tick Ixodes persulcatus Schulze

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationHokkaido Universityen_US
cg.contributor.affiliationHokkaido Institute of Public Healthen_US
cg.contributor.affiliationNational Institute of Infectious Diseases, Japanen_US
cg.contributor.affiliationUniversidade Federal do Rio de Janeiroen_US
cg.contributor.affiliationUniversidade Federal do Rio Grande do Sulen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorJapan Agency for Medical Research and Developmenten_US
cg.contributor.donorJapan Society for the Promotion of Scienceen_US
cg.contributor.donorCoordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazilen_US
cg.creator.identifierNaftaly Githaka: 0000-0003-4530-7164en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.ttbdis.2019.101332en_US
cg.isijournalISI Journalen_US
cg.issn1877-959Xen_US
cg.issue2en_US
cg.journalTicks and Tick-borne Diseasesen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.volume11en_US
dc.contributor.authorSajiki, Y.en_US
dc.contributor.authorKonnai, S.en_US
dc.contributor.authorOchi, A.en_US
dc.contributor.authorOkagawa, T.en_US
dc.contributor.authorGithaka, Naftaly W.en_US
dc.contributor.authorIsezaki, M.en_US
dc.contributor.authorYamada, S.en_US
dc.contributor.authorIto, T.en_US
dc.contributor.authorAndo, S.en_US
dc.contributor.authorKawabata, H.en_US
dc.contributor.authorLogullo, C.en_US
dc.contributor.authorSilva Vaz, I. daen_US
dc.contributor.authorMaekawa, N.en_US
dc.contributor.authorMurata, S.en_US
dc.contributor.authorOhashi, K.en_US
dc.date.accessioned2020-09-08T15:50:39Zen_US
dc.date.available2020-09-08T15:50:39Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/109373en_US
dc.titleImmunosuppressive effects of sialostatin L1 and L2 isolated from the taiga tick Ixodes persulcatus Schulzeen_US
dcterms.abstractTick saliva contains immunosuppressants which are important to obtain a blood meal and enhance the infectivity of tick-borne pathogens. In Japan, Ixodes persulcatus is a major vector for Lyme borreliosis pathogens, such as Borrelia garinii, as well as for those causing relapsing fever, such as B. miyamotoi. To date, little information is available on bioactive salivary molecules, produced by this tick. Thus, in this study, we identified two proteins, I. persulcatus derived sialostatin L1 (Ip-sL1) and sL2 (Ip-sL2), as orthologs of I. scapularis derived sL1 and sL2. cDNA clones of Ip-sL1 and Ip-sL2 shared a high identity with sequences of sL1 and sL2 isolated from the salivary glands of I. scapularis. Semi-quantitative PCR revealed that Ip-sL1 and Ip-sL2 were expressed in the salivary glands throughout the life of the tick. In addition, Ip-sL1 and Ip-sL2 were expressed even before the ticks started feeding, and their expression continued during blood feeding. Recombinant Ip-sL1 and Ip-sL2 were developed to characterize the proteins via biological and immunological analyses. These analyses revealed that both Ip-sL1 and Ip-sL2 had inhibitory effects on cathepsins L and S. Ip-sL1 and Ip-sL2 inhibited the production of IP-10, TNFα, and IL-6 by LPS-stimulated bone-marrow-derived dendritic cells (BMDCs). Additionally, Ip-sL1 significantly impaired BMDC maturation. Taken together, these results suggest that Ip-sL1 and Ip-sL2 confer immunosuppressive functions and appear to be involved in the transmission of pathogens by suppressing host immune responses, such as cytokine production and dendritic cell maturation. Therefore, further studies are warranted to investigate the immunosuppressive functions of Ip-sL1 and Ip-sL2 in detail to clarify their involvement in pathogen transmission via I. persulcatus.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationSajiki, Y., Konnai, S., Ochi, A., Okagawa, T., Githaka, N., Isezaki, M., Yamada, S., Ito, T., Ando, S., Kawabata, H., Logullo, C., Silva Vaz Jr., I. da, Maekawa, N., Murata, S. and Ohashi, K. 2020. Immunosuppressive effects of sialostatin L1 and L2 isolated from the taiga tick Ixodes persulcatus Schulze. Ticks and Tick-borne Diseases 11(2): 101332.en_US
dcterms.issued2020-03en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherElsevieren_US
dcterms.subjecttickborne diseasesen_US
dcterms.typeJournal Articleen_US

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