Usage of FTA® Classic cards for safe storage, shipment, and detection of arboviruses

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationUppsala Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationSwedish University of Agricultural Sciencesen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorSwedish Research Council for Environment, Agricultural Sciences and Spatial Planningen
cg.contributor.donorSciLifeLab Pandemic Preparednessen
cg.contributor.donorEuropean Unionen
cg.contributor.donorBörjesson E o R stipendsen
cg.creator.identifierJohanna Lindahl: 0000-0002-1175-0398en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3390/microorganisms10071445en
cg.isijournalISI Journalen
cg.issn2076-2607en
cg.issue7en
cg.journalMicroorganismsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriHUMAN HEALTHen
cg.subject.ilriRESEARCHen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.volume10en
dc.contributor.authorKrambrich, J.en
dc.contributor.authorBringeland, E.en
dc.contributor.authorHesson, J.C.en
dc.contributor.authorHoffman, T.en
dc.contributor.authorLundkvist, Å.en
dc.contributor.authorLindahl, Johanna F.en
dc.contributor.authorLing, J.en
dc.date.accessioned2022-07-27T12:22:35Zen
dc.date.available2022-07-27T12:22:35Zen
dc.identifier.urihttps://hdl.handle.net/10568/120340
dc.titleUsage of FTA® Classic cards for safe storage, shipment, and detection of arbovirusesen
dcterms.abstractInfections caused by arthropod-borne RNA viruses are overrepresented among emerging infectious diseases. Effective methods for collecting, storing, and transporting clinical or biological specimens are needed worldwide for disease surveillance. However, many tropical regions where these diseases are endemic lack analytical facilities and possibility of continuous cold chains, which presents challenges from both a biosafety and material preservation perspective. Whatman® FTA® Classic Cards may serve as an effective and safe option for transporting hazardous samples at room temperature, particularly for RNA viruses classified as biosafety level (BSL) 2 and 3 pathogens, from sampling sites to laboratories. In this study, we investigated the biosafety and perseverance of representative alpha- and flaviviruses stored on FTA® cards. To evaluate the virus inactivation capacity of FTA® cards, we used Sindbis virus (SINV), chikungunya virus (CHIKV), and Japanese encephalitis virus (JEV). We inoculated susceptible cells with dilution series of eluates from viral samples stored on the FTA® cards and observed for cytopathic effect to evaluate the ability of the cards to inactivate viruses. All tested viruses were inactivated after storage on FTA® cards. In addition, we quantified viral RNA of JEV, SINV, and tick-borne encephalitis virus (TBEV) stored on FTA® cards at 4 °C, 25 °C, and 37 °C for 30 days using two reverse transcriptase quantitative PCR assays. Viral RNA of SINV stored on FTA® cards was not reduced at either 4 °C or 25 °C over a 30-day period, but degraded rapidly at 37 °C. For JEV and TBEV, degradation was observed at all temperatures, with the most rapid degradation occurring at 37 °C. Therefore, the use of FTA® cards provides a safe and effective workflow for the collection, storage, and analysis of BSL 2- and 3-virus RNA samples, but there is a risk of false negative results if the cards are stored at higher temperatures for long periods of time. Conscious usage of the cards can be useful in disease surveillance and research, especially in tropical areas where transportation and cold chains are problematic.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2022-07-18en
dcterms.bibliographicCitationKrambrich, J., Bringeland, E., Hesson, J.C., Hoffman, T., Lundkvist, Å., Lindahl, J.F. and Ling, J. 2022. Usage of FTA® Classic cards for safe storage, shipment, and detection of arboviruses. Microorganisms 10(7): 1445.en
dcterms.extent1445en
dcterms.issued2022-07-18en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherMDPIen
dcterms.subjectarbovirusesen
dcterms.subjectbiosafetyen
dcterms.subjectflavivirusen
dcterms.subjecthealthen
dcterms.subjectresearchen
dcterms.typeJournal Article

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