Effect of Gas Exchange Rate, Vessel Type, Planting Density, and Genotype on Growth, Photosynthetic Activity, and Ion Uptake of In Vitro Potato Plants

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cg.authorship.typesCGIAR single centreen_US
cg.contributor.affiliationInternational Potato Centeren_US
cg.contributor.donorGlobal Crop Diversity Trusten_US
cg.creator.identifierRainer Vollmer: 0000-0001-8428-2241en_US
cg.creator.identifierJaneth Espirilla: 0000-0002-8786-0606en_US
cg.creator.identifierAna Belén Espinoza Jara: https://orcid.org/0000-0002-1623-2389en_US
cg.identifier.doihttps://doi.org/10.3390/plants13192830en_US
cg.isijournalISI Journalen_US
cg.issn2223-7747en_US
cg.issue19en_US
cg.journalPlantsen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.cipPOTATO AGRI-FOOD SYSTEMSen_US
cg.subject.cipPOTATOESen_US
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCIen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.subject.sdgSDG 2 - Zero Hungeren_US
cg.subject.sdgSDG 12 - Responsible Consumption and Productionen_US
cg.subject.sdgSDG 15 - Life on Landen_US
cg.volume13en_US
dc.contributor.authorVollmer, R.en_US
dc.contributor.authorEspirilla, J.en_US
dc.contributor.authorEspinoza, A.en_US
dc.contributor.authorVillagaray, R.en_US
dc.contributor.authorCastro, M.en_US
dc.contributor.authorPineda, S.en_US
dc.contributor.authorSánchez, J.C.en_US
dc.contributor.authorMello, A.F.S.en_US
dc.contributor.authorAzevedo, V.C.R.en_US
dc.date.accessioned2024-10-15T20:15:57Zen_US
dc.date.available2024-10-15T20:15:57Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/155362en_US
dc.titleEffect of Gas Exchange Rate, Vessel Type, Planting Density, and Genotype on Growth, Photosynthetic Activity, and Ion Uptake of In Vitro Potato Plantsen_US
dcterms.abstractThe growth of high-quality in vitro potato plants (Solanum stenotomum subsp. stenotomum, Solanum stenotomum subsp. goniocalyx, and Solanum tuberosum subsp. andigena) is affected by multiple biological, operational, and environmental factors. Research on in vitro culture is frequently focused on the species, explant, composition of the culture medium, and incubation conditions, but only limited information is available on the effect of the gas exchange rate and volume of in vitro culture vessels under variable planting densities. In the present study, these factors were evaluated with a set of seven diverse potato landraces. The results were compared to the plants’ responses in routinely used in vitro culture vessels, i.e., 13 × 100 mm and 25 × 150 mm test tubes, and GA7® magenta vessels. In vitro potato plants grown in plastic vessels equipped with a HEPA filter delivering a high gas exchange rate developed thicker stems (0.95 mm), a higher total average leaf area (2.51 cm2), increased chlorophyll content in leaves (32.2 ppm), and lower moisture content in their tissues (90.1%) compared to filter systems with lower gas exchange rates. A high planting density of 10 × 10 plants per vessel (360 and 870 mL) negatively affected the average stem width and root length but increased the plant height (3.4 cm). High fluctuations of ion-uptake of NO3−, Ca++, K+, and Na+ were observed between genotypes, with some accessions having a 4.6-times higher Ca++-ion concentration in their tissues (190–234 ppm). The in vitro plants developed more robust stems, longer roots, and larger leaves within in vitro culture vessels equipped with a HEPA filter (high gas exchange rate) compared to the control vessels, in contrast to the chlorophyll content in leaves, which was higher in plants grown in narrow test tubes. Depending on the purpose of the subculture of in vitro plants, their growth and development can be molded using different gas exchange rates, planting densities, and vessel volumes.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceAcademicsen_US
dcterms.audienceCGIARen_US
dcterms.audienceDevelopment Practitionersen_US
dcterms.audienceDonorsen_US
dcterms.audienceExtensionen_US
dcterms.audienceFarmersen_US
dcterms.audienceGeneral Publicen_US
dcterms.audienceNGOsen_US
dcterms.audiencePolicy Makersen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationVollmer, R.; Espirilla, J.; Espinoza, A.; Villagaray, R.; Castro, M.; Pineda, S.; Sánchez, J.C.; Mello, A.F.S.; Azevedo, V.C.R. 2024. Effect of Gas Exchange Rate, Vessel Type, Planting Density, and Genotype on Growth, Photosynthetic Activity, and Ion Uptake of In Vitro Potato Plants. Plants. ISSN 2223-7747. 13(19), 2830. https://doi.org/10.3390/plants13192830en_US
dcterms.issued2024-10-09en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherMDPI AGen_US
dcterms.subjectSolanumen_US
dcterms.subjectleaf areaen_US
dcterms.subjectstem lengthen_US
dcterms.subjectpotatoesen_US
dcterms.subjectplant growthen_US
dcterms.subjectcrop improvementen_US
dcterms.subjectgenetic resourcesen_US
dcterms.typeJournal Articleen_US

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