Characterization of the Theileria parva sporozoite proteome

Simple item page
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationWageningen University & Researchen_US
cg.contributor.affiliationUniversity of Oxforden_US
cg.contributor.affiliationUniversity of Marylanden_US
cg.contributor.affiliationSouth Australian Health and Medical Research Instituteen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorUnited States Agency for International Developmenten_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.ijpara.2017.09.007en_US
cg.isijournalISI Journalen_US
cg.issn0020-7519en_US
cg.issue3-4en_US
cg.journalInternational Journal for Parasitologyen_US
cg.reviewStatusPeer Reviewen_US
cg.speciesTheileria parvaen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriVACCINESen_US
cg.volume48en_US
dc.contributor.authorNyagwange, Jamesen_US
dc.contributor.authorTijhaar, Edwinen_US
dc.contributor.authorTernette, Nicolaen_US
dc.contributor.authorMobegi, Fredricken_US
dc.contributor.authorTretina, Kyleen_US
dc.contributor.authorSilva, Joana C.en_US
dc.contributor.authorPelle, Rogeren_US
dc.contributor.authorNene, Vishvanath M.en_US
dc.date.accessioned2018-01-03T16:35:58Zen_US
dc.date.available2018-01-03T16:35:58Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/89911en_US
dc.titleCharacterization of the Theileria parva sporozoite proteomeen_US
dcterms.abstractEast Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC–MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50% of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationNyagwange, J., Tijhaar, E., Ternette, N., Mobegi, F., Tretina, K., Silva, J.C., Pelle, R. and Nene, V. 2018. Characterization of the Theileria parva sporozoite proteome. International Journal for Parasitology 48(3-4):265-273.en_US
dcterms.extentp. 265-273en_US
dcterms.issued2018-03en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherElsevieren_US
dcterms.subjecttheileriaen_US
dcterms.subjectcattleen_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectvaccinesen_US
dcterms.subjectdisease controlen_US
dcterms.subjecteast coast feveren_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
CRP Livestock health flagship
CRP Livestock journal articles
ILRI animal and human health program outputs (2017-2024)
ILRI BecA articles
ILRI BecA outputs
Load more