Developing a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrification

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInstituto Nacional de Investigaciones Agropecuarias, Ecuadoren
cg.contributor.donorFondo de Investigación para la Agrobiodiversidad, Semillas y Agricultura Sostenible, Ecuadoren
cg.coverage.countryEcuador
cg.coverage.iso3166-alpha2EC
cg.coverage.regionSouth America
cg.creator.identifierTacán Marcelo: 0000-0002-6775-1445en
cg.creator.identifierRainer Vollmer: 0000-0001-8428-2241en
cg.creator.identifiercesar tapia: 0000-0002-9844-4595en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3390/crops4040048en
cg.isijournalISI Journalen
cg.issn2673-7655en
cg.issue4en
cg.journalCropsen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.cipBIODIVERSITY FOR THE FUTUREen
cg.subject.cipCRYOPRESERVATIONen
cg.subject.cipGENEBANKen
cg.subject.cipGENETIC RESOURCESen
cg.subject.cipCROP AND SYSTEMS SCIENCES CSSen
cg.subject.impactAreaEnvironmental health and biodiversity
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.sdgSDG 15 - Life on landen
cg.volume4en
dc.contributor.authorTacán, M.en
dc.contributor.authorAndrango, R.en
dc.contributor.authorTapia, C.en
dc.contributor.authorSørensen, M.en
dc.contributor.authorVollmer, R.en
dc.contributor.authorPérez, C.en
dc.date.accessioned2025-01-13T16:57:25Zen
dc.date.available2025-01-13T16:57:25Zen
dc.identifier.urihttps://hdl.handle.net/10568/168916
dc.titleDeveloping a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrificationen
dcterms.abstractThe present study investigates the cryopreservation of embryonic axes from the peanut genotype ECU-12466, demonstrating a successful protocol involving a 1 h desiccation on silica gel followed by a 1 h cryoprotection with Plant Vitrificatin Solution 2 (PVS2). The average dimensions of the excised embryonic axes were 5.6 mm in length and 3.5 mm in width, with plumule lengths averaging 2.2 mm. Notably, germination rates for cryopreserved axes ranged from 71.4% to 85.7%, showing resilience to varying desiccation and PVS2 treatment times, particularly at 1 h. Shoot length was significantly enhanced by a 1 h PVS2 exposure, while longer durations resulted in phytotoxic effects. Rooting rates were higher for samples treated with shorter desiccation periods, with 54% rooting achieved at 1 h of PVS2 exposure, contrasting sharply with just 16% at 2 h. The moisture content of the embryonic axes remained stable between 9.3% and 9.5%, indicating no detrimental impact from the applied treatments. To evaluate the protocol’s broader applicability, five additional peanut genotypes (ECU-11401, ECU-11418, ECU-11448, ECU-11469, and ECU-11494) were tested. While cryopreserved samples demonstrated high germination rates of up to 95.4% after 15 days, the rooting success was significantly lower (25.2%) compared to the control group’s 90.9%. Following transplantation, the growth performance varied among genotypes, with a success rate of 93% for ECU-11494. Overall, this study elucidates the critical parameters for optimizing cryopreservation protocols in peanut embryonic axes, contributing to more effective long-term conservation strategies.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.audienceFarmersen
dcterms.audienceGeneral Publicen
dcterms.audienceNGOsen
dcterms.audiencePolicy Makersen
dcterms.audienceScientistsen
dcterms.bibliographicCitationTacán, M.; Andrango, R.; Tapia, C.; Sørensen, M.; Vollmer, R.; Pérez, C. 2024. Developing a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrification. Crops. ISSN 2673-7655. 4(4), 701–716. https://doi.org/10.3390/crops4040048en
dcterms.issued2024-12-23en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.subjectgroundnutsen
dcterms.subjectdehydrationen
dcterms.typeJournal Article

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