Developing a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrification.

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInstituto Nacional de Investigaciones Agropecuarias, Ecuadoren_US
cg.coverage.regionSouth Americaen_US
cg.creator.identifierTacán Marcelo: 0000-0002-6775-1445en_US
cg.creator.identifierRainer Vollmer: 0000-0001-8428-2241en_US
cg.creator.identifiercesar tapia: 0000-0002-9844-4595en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.3390/crops4040048en_US
cg.isijournalISI Journalen_US
cg.issn2673-7655en_US
cg.issue4en_US
cg.journalCropsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.cipBIODIVERSITY FOR THE FUTUREen_US
cg.subject.cipCRYOPRESERVATIONen_US
cg.subject.cipGENEBANKen_US
cg.subject.cipGENETIC RESOURCESen_US
cg.subject.cipCROP AND SYSTEMS SCIENCES CSSen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.subject.impactAreaClimate adaptation and mitigationen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 13 - Climate actionen_US
cg.subject.sdgSDG 15 - Life on landen_US
cg.volume4en_US
dc.contributor.authorTacán, M.en_US
dc.contributor.authorAndrango, R.en_US
dc.contributor.authorTapia, C.en_US
dc.contributor.authorSørensen, M.en_US
dc.contributor.authorVollmer, R.en_US
dc.contributor.authorPérez, C.en_US
dc.date.accessioned2025-01-13T16:57:25Zen_US
dc.date.available2025-01-13T16:57:25Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/168916en_US
dc.titleDeveloping a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrification.en_US
dcterms.abstractThe present study investigates the cryopreservation of embryonic axes from the peanut genotype ECU-12466, demonstrating a successful protocol involving a 1 h desiccation on silica gel followed by a 1 h cryoprotection with Plant Vitrificatin Solution 2 (PVS2). The average dimensions of the excised embryonic axes were 5.6 mm in length and 3.5 mm in width, with plumule lengths averaging 2.2 mm. Notably, germination rates for cryopreserved axes ranged from 71.4% to 85.7%, showing resilience to varying desiccation and PVS2 treatment times, particularly at 1 h. Shoot length was significantly enhanced by a 1 h PVS2 exposure, while longer durations resulted in phytotoxic effects. Rooting rates were higher for samples treated with shorter desiccation periods, with 54% rooting achieved at 1 h of PVS2 exposure, contrasting sharply with just 16% at 2 h. The moisture content of the embryonic axes remained stable between 9.3% and 9.5%, indicating no detrimental impact from the applied treatments. To evaluate the protocol’s broader applicability, five additional peanut genotypes (ECU-11401, ECU-11418, ECU-11448, ECU-11469, and ECU-11494) were tested. While cryopreserved samples demonstrated high germination rates of up to 95.4% after 15 days, the rooting success was significantly lower (25.2%) compared to the control group’s 90.9%. Following transplantation, the growth performance varied among genotypes, with a success rate of 93% for ECU-11494. Overall, this study elucidates the critical parameters for optimizing cryopreservation protocols in peanut embryonic axes, contributing to more effective long-term conservation strategies.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceAcademicsen_US
dcterms.audienceCGIARen_US
dcterms.audienceDevelopment Practitionersen_US
dcterms.audienceDonorsen_US
dcterms.audienceExtensionen_US
dcterms.audienceFarmersen_US
dcterms.audienceGeneral Publicen_US
dcterms.audienceNGOsen_US
dcterms.audiencePolicy Makersen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationTacán, M.; Andrango, R.; Tapia, C.; Sørensen, M.; Vollmer, R.; Pérez, C. 2024. Developing a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation–Vitrification. Crops. ISSN 2673-7655. 4(4), 701–716. https://doi.org/10.3390/crops4040048en_US
dcterms.issued2024-12-23en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.subjectgroundnutsen_US
dcterms.subjectdehydrationen_US
dcterms.typeJournal Articleen_US

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