The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationFriedrich Loeffler Instituten
cg.contributor.affiliationUniversity of Nottinghamen
cg.contributor.affiliationUniversity of Nairobien
cg.contributor.crpLivestocken
cg.contributor.donorInternational Development Research Centreen
cg.contributor.donorGlobal Alliance for Livestock Veterinary Medicinesen
cg.coverage.countryKenyaen
cg.coverage.iso3166-alpha2KEen
cg.coverage.regionAfricaen
cg.coverage.regionEastern Africaen
cg.creator.identifierJohanneke Dinie Hemmink: 0000-0001-8764-4156en
cg.creator.identifierHussein Abkallo: 0000-0002-5594-4418en
cg.creator.identifierSonal P Henson: 0000-0002-2002-5462en
cg.creator.identifierEmmanuel Mulaya Khazalwa: 0000-0001-6233-483Xen
cg.creator.identifierVictor Riitho: 0000-0002-0624-0115en
cg.creator.identifierLucilla Steinaa: 0000-0003-3691-3971en
cg.creator.identifierAbworo: 0000-0003-0689-823Xen
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970en
cg.creator.identifierAnna Lacasta: 0000-0001-8381-9844en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3390/v14091912en
cg.isijournalISI Journalen
cg.issn1999-4915en
cg.issue9en
cg.journalVirusesen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriASFen
cg.subject.ilriPIGSen
cg.subject.impactAreaNutrition, health and food securityen
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume14en
dc.contributor.authorHemmink, Johanneke D.en
dc.contributor.authorAbkallo, Hussein M.en
dc.contributor.authorHenson, Sonal P.en
dc.contributor.authorKhazalwa, Emmanuel M.en
dc.contributor.authorOduor, Bernarden
dc.contributor.authorLacasta, Annaen
dc.contributor.authorOkoth, Edward A.en
dc.contributor.authorRiitho, Victoren
dc.contributor.authorFuchs, W.en
dc.contributor.authorBishop, Richard P.en
dc.contributor.authorSteinaa, Lucillaen
dc.date.accessioned2022-09-02T10:52:57Zen
dc.date.available2022-09-02T10:52:57Zen
dc.identifier.urihttps://hdl.handle.net/10568/121075
dc.titleThe African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSLen
dcterms.abstractWe describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during a reported outbreak. This includes the efficiency of virus replication and in vivo virulence, together with genome stability and virulence, following passage in blood macrophages and in a wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate in primary macrophages and retained virulence in vivo, following more than 20 passages in a WSL. At the whole genome level, a few single-nucleotide differences were observed between the macrophage and WSL-propagated viruses. Thus, we propose that the WSL is suitable for the production of live-attenuated ASFV vaccine candidates based on the modification of this wild-type isolate. The genome sequences for ASFV-Kenya-IX-1033 propagated in macrophages and in WSL cells were submitted to GenBank, and a challenge model based on the isolate was developed. This will aid the development of vaccines against the genotype IX ASFV circulating in eastern and central Africa.en
dcterms.accessRightsOpen Accessen
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2022-08-29en
dcterms.bibliographicCitationHemmink, J.D., Abkallo, H.M., Henson, S.P., Khazalwa, E.M., Oduor, B., Lacasta, A., Okoth, E., Riitho, V., Fuchs, W., Bishop, R.P. and Steinaa, L. 2022. The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL. Viruses 14(9): 1912.en
dcterms.extent1912en
dcterms.issued2022-08-29en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherMDPIen
dcterms.subjectafrican swine fever virusen
dcterms.subjectanimal diseasesen
dcterms.subjectswineen
dcterms.typeJournal Articleen

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