A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationNanjing Agricultural Universityen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.donorNational Key Research and Development Programen
cg.coverage.countryChina
cg.coverage.iso3166-alpha2CN
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1186/s42483-024-00284-3en
cg.isijournalISI Journalen
cg.issn2524-4167en
cg.issue1en
cg.journalPhytopathology Researchen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.cipPOTATOESen
cg.subject.cipCROP PROTECTIONen
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCIen
cg.subject.cipFOOD SECURITYen
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.subject.sdgSDG 12 - Responsible consumption and productionen
cg.subject.sdgSDG 15 - Life on landen
cg.volume6en
dc.contributor.authorZhang, X.en
dc.contributor.authorChen, H.en
dc.contributor.authorWu, J.en
dc.contributor.authorWang, X.en
dc.contributor.authorWang, S.en
dc.contributor.authorChen, J.en
dc.contributor.authorYu, Q.en
dc.contributor.authorZhang, Z.en
dc.contributor.authorZheng, X.en
dc.contributor.authorZeng, J.en
dc.contributor.authorDong, S.en
dc.date.accessioned2025-03-10T20:15:38Zen
dc.date.available2025-03-10T20:15:38Zen
dc.identifier.urihttps://hdl.handle.net/10568/173551
dc.titleA field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assaysen
dcterms.abstractVarious pathogens from oomycete, fungi, and bacteria kingdoms can infect potato and significantly reduce potato yield. The early diagnosis of potato pathogens is important for tracing disease epidemics and the subsequent disease management. Loop-mediated isothermal amplification (LAMP) is a critical technique for pathogen detection, but available LAMP assays do not effectively meet the requirement of field diagnoses due to complexities including co-infection of different pathogens. Hence, this study aims to develop integrated-LAMP assays (iLAMPs) for simultaneous detection of eight common potato pathogens and apply iLAMPs to pathogen detection in field samples from the four main potato-growing regions of China in 2023. Therefore, eight sets of primers showing gene- and genus-specificity were designed and used for iLAMPs to determine their specificity, sensitivity, and visualization. Subsequently, iLAMPs-mediated pathogen detection revealed that 72.82% of 206 diseased leaves and 84.94% of 239 diseased tubers carry more than one pathogen. The detection rate for each pathogen significantly varies from 1.94 to 65.53% in diseased leaves, and ranges from 26.78 to 52.72% in diseased tubers, respectively. In addition, the detection rate of Phytophthora infestans and Alternaria solani positively correlates in both leaves and tubers, especially for those samples from the southwestern and southern regions. Taken together, iLAMPs developed in this study enables simultaneous detection of eight common potato pathogens from field samples and may have broad applications in early management of potato diseases.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.audienceFarmersen
dcterms.audienceGeneral Publicen
dcterms.audienceNGOsen
dcterms.audiencePolicy Makersen
dcterms.audienceScientistsen
dcterms.bibliographicCitationZhang, X.; Chen, H.; Wu, J.; Wang, X.; Wang, S.; Chen, J.; Yu, Q.; Zhang, Z.; Zheng, X.; Zeng, J.; Dong, S. 2024. A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays. Phytopathology Research. ISSN 2524-4167. 6(1). https://doi.org/10.1186/s42483-024-00284-3en
dcterms.extent14 p.en
dcterms.issued2024-12-10en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.subjectsolanum tuberosumen
dcterms.subjectpotatoesen
dcterms.subjectplant protectionen
dcterms.typeJournal Article

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