Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique

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cg.authorship.typesCGIAR single centreen
cg.contributor.affiliationEmpresa Brasileira de Pesquisa Agropecuáriaen
cg.contributor.affiliationMugla Sitki Kocman Universityen
cg.contributor.affiliationState University of Feira de Santanaen
cg.contributor.affiliationFederal University of Recôncavo da Bahiaen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.affiliationUnited States Department of Agricultureen
cg.contributor.donorUnited States Department of Agricultureen
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.scienta.2017.06.042en
cg.isijournalISI Journalen
cg.issn0304-4238en
cg.journalScientia Horticulturaeen
cg.reviewStatusPeer Reviewen
cg.subject.cipCRYOPRESERVATIONen
cg.subject.cipBIODIVERSITY FOR THE FUTUREen
cg.volume224en
dc.contributor.authorDuarte Souza, F.V.en
dc.contributor.authorKaya, E.en
dc.contributor.authorVieira, L.J.en
dc.contributor.authorSilva Souza, A.en
dc.contributor.authorSilva Carvalho, M.J.en
dc.contributor.authorBarbosa Santos, E.en
dc.contributor.authorCunha Alves, A.A.en
dc.contributor.authorEllis, Daviden
dc.date.accessioned2017-09-01T14:25:49Zen
dc.date.available2017-09-01T14:25:49Zen
dc.identifier.urihttps://hdl.handle.net/10568/83307
dc.titleCryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate techniqueen
dcterms.abstractA modified aluminum cryo-plate technique was applied for cryopreservation of embryogenic citrus calli using aluminum cryo-plate. After encapsulation in a medium with 3% alginate, the beads were placed directly on aluminum foil troughs for exposure to plant vitrification solution (PVS2) incubated on ice for three intervals (30, 45 and 60 min). After rewarming, the calli were incubated under light (16 h/8 h) or dark at 27 ± 2 °C. The survival rates ranged from 59.2% following 45 min of PVS2 exposure to 88.7% after 30 min of exposure. The 30-min PVS2 treatment yielded the highest callus growth (1.4) and percentage of calli with embryos regenerated (43.57%). The highest percentage of embryos regenerated was observed in dark condition (75.2%). The method described using aluminum foil troughs is efficient for cryopreservation of embryogenic citrus calli.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationDuarte Souza, F.V.; Kaya, E.; Vieira, L.J.; Silva Souza, A.; Silva Carvalho, M.J.; Barbosa Santos, E.; Cunha Alves, A.A.; Ellis, D. 2017. Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae. (Netherlands). ISSN 0304-4238. 224:302-305.en
dcterms.extent302-305en
dcterms.issued2017-10
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectcryopreservationen
dcterms.subjecttechniquesen
dcterms.subjectsomatic embryogenesisen
dcterms.typeJournal Article

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