B-cell activation in trypanosome-infected Boran cattle

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cg.identifier.urlhttps://hdl.handle.net/10568/2726en
cg.isbn92-9055-299-9en
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriLIVESTOCKen
dc.contributor.authorTaylor, K.A.en
dc.contributor.authorWilliams, D.J.L.en
dc.contributor.authorLutje, V.en
dc.date.accessioned2010-12-09T11:11:59Zen
dc.date.available2010-12-09T11:11:59Zen
dc.identifier.urihttps://hdl.handle.net/10568/2825
dc.titleB-cell activation in trypanosome-infected Boran cattleen
dcterms.abstractAnalysis of serum antibody responses in cattle experimentally infected with Trypanosoma congolense suggested that antibody responses of trypanosusceptible Boran cattle are less efficient in isotype switching and affinity maturation than those of trypanotolerant N'Dama cattle. High levels of polyspecific IgM are produced by Boran cattle while N'Dama cattle have higher titres of IgG specific for certain trypanosome antigens. Further characterization of these differences required determination of the frequency and phenotype of antibody secreting cells (ASC), as well as the specificity of the antibody and the isotype secreted in response to infection. A method was therefore developed to maintain bovine B cells in culture and stimulate them to differentiate into ASC. B cells were examined for the expression of the transferrin receptor and the CD5 antigen using fluorescence activated cell sorter (FACS) analysis and the number of ASC was determined using an ELISpot assay. We compared B-cell activation and the frequency of ASC between six Trypanosoma congolense-infected cattle and three uninfected controls. Peripheral blood lymphocytes (PBL) were analysed before and after in vitro culture for three and six days with combinations of lipopolysaccharide (LPS), pokeweed mitogen (PWM) and recombinant bovine interleukin-2 (rboIL 2). Splenic lymphocytes from T. congolense-infected cattle were compared with PBL from the same animals.en
dcterms.accessRightsOpen Access
dcterms.issued1995
dcterms.languageen
dcterms.licenseOther
dcterms.publisherInternational Laboratory for Research on Animal Diseasesen
dcterms.subjecttrypanosomiasisen
dcterms.subjectanimal diseasesen
dcterms.subjectlymphocytesen
dcterms.subjectantibodiesen
dcterms.subjectimmune responseen
dcterms.subjectdisease controlen
dcterms.subjectglossinidaeen
dcterms.subjectndama cattleen
dcterms.subjecttrypanosoma congolenseen
dcterms.typeConference Paper

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