Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversité de la Manoubaen
cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen
cg.contributor.crpDryland Systemsen
cg.contributor.crpLivestocken
cg.coverage.countryTunisiaen
cg.coverage.iso3166-alpha2TNen
cg.coverage.regionAfricaen
cg.coverage.regionNorthern Africaen
cg.creator.identifierMourad Rekik: 0000-0001-7455-2017en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1002/vms3.53en
cg.isijournalISI Journalen
cg.issn2053-1095en
cg.issue1en
cg.journalVeterinary Medicine and Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL PRODUCTSen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriRESEARCHen
cg.subject.ilriSHEEPen
cg.volume3en
dc.contributor.authorRouatbi, M.en
dc.contributor.authorAmdouni, Y.en
dc.contributor.authorAmairia, S.en
dc.contributor.authorRjeibi, M.R.en
dc.contributor.authorSammoudi, S.en
dc.contributor.authorRekik, Mouraden
dc.contributor.authorGharbi, M.en
dc.date.accessioned2018-05-25T14:03:51Zen
dc.date.available2018-05-25T14:03:51Zen
dc.identifier.urihttps://hdl.handle.net/10568/92921
dc.titleMolecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisiaen
dcterms.abstractToxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan Toxoplasma gondii. It can infect all warm-blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with T. gondii in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of T. gondii. Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja. DNA was extracted from the samples using the Wizard® genomic DNA purification kit. A nested PCR using two pairs of primers (NN 1 and NN2, Tg-NP1 and Tg-NP2) were used to detect infection with T. gondii, which was then confirmed by sequencing. Eight T. gondii amplicons were sequenced (accession number KT896498) and deposited in GenBank. The T. gondii amplicons showed 97–100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested PCR detected T. gondii DNA in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of T. gondii infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.en
dcterms.accessRightsOpen Accessen
dcterms.audienceScientistsen
dcterms.available2016-12-20en
dcterms.bibliographicCitationRouatbi, M., Amdouni, Y., Amairia, S., Rjeibi, M.R., Sammoudi, S., Rekik, M. and Gharbi, M. 2017. Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia. Veterinary Medicine and Science 3(1): 22-31en
dcterms.extentpp. 22-31en
dcterms.issued2017-02en
dcterms.languageenen
dcterms.licenseCC-BY-NC-4.0en
dcterms.publisherWileyen
dcterms.subjectlivestocken
dcterms.subjectsheepen
dcterms.subjectanimal productsen
dcterms.typeJournal Articleen

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