Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures

Simple item page
cg.contributor.affiliationJapan International Research Center for Agricultural Sciencesen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.creator.identifierJan Naessens: 0000-0002-7075-9915
cg.creator.identifierJohn Gibson: 0000-0003-0371-2401
cg.identifier.doihttps://doi.org/10.1128/iai.70.4.2210-2214.2002en
cg.issn0019-9567en
cg.issue4en
cg.journalInfection and Immunityen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriGENETICSen
cg.subject.ilriLIVESTOCKen
cg.volume70en
dc.contributor.authorKitani, H.en
dc.contributor.authorBlack, Samuel J.en
dc.contributor.authorNakamura, Y.en
dc.contributor.authorNaessens, Janen
dc.contributor.authorMurphy, N.en
dc.contributor.authorYokomizo, Y.en
dc.contributor.authorGibson, John P.en
dc.contributor.authorIraqi, F.A.en
dc.date.accessioned2010-05-17T06:53:59Zen
dc.date.available2010-05-17T06:53:59Zen
dc.identifier.urihttps://hdl.handle.net/10568/1540
dc.titleRecombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic culturesen
dcterms.abstractMice whose tumor necrosis factor alpha (TNF-) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with Trypanosoma congolense IL1180 or T. brucei brucei GUTat3.1, confirming the results of earlier studies. To determine whether TNF- directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF- on the growth of two isolates of T. congolense, IL1180 and IL3338, and two isolates of T. brucei brucei, GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF- used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF- lots tested, one lot of mouse TNF- inhibited the growth of both isolates of T. brucei brucei and one lot of bovine TNF- inhibited the growth of T. brucei brucei ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-, as well as human TNF-, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-/ml of medium). These results suggest that exogenously added recombinant TNF- generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF- on trypanosome parasitemia may be indirect, at least with respect to the four strains of trypanosomes reported here.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationKitani, H.; Black, S.J.; Nakamura, Y.; Naessens, J.; Murphy, N.; Yokomizo, Y.; Gibson, J.; Iraqi, F. 2002. Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures. Infection and Immunology 70(4):2210-2214.en
dcterms.extentp. 2210-2214en
dcterms.issued2002-04
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherAmerican Society for Microbiologyen
dcterms.subjecttrypanosoma congolenseen
dcterms.subjectanimal diseasesen
dcterms.typeJournal Article

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.79 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
ILRI animal bioscience program outputs