The impact of translocation mechanisms and plant architecture on the success or failure of propagation, a thidiazuron case study

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Center for Tropical Agricultureen_US
cg.contributor.affiliationResearch Institute for Nature and Foresten_US
cg.contributor.donorEuropean Cooperation in Science and Technologyen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeGenebanksen_US
cg.coverage.countryLatviaen_US
cg.coverage.iso3166-alpha2LVen_US
cg.coverage.regionEuropeen_US
cg.coverage.regionNorthern Europeen_US
cg.creator.identifierHannes Wilms: 0000-0001-8671-3447en_US
cg.creator.identifierBart Panis: 0000-0001-6717-947Xen_US
cg.identifier.dataurlhttps://a92860b9-c35d-489e-93dc-4dd93978ee6e.usrfien_US
cg.identifier.urlhttps://www.copytree.eu/en_US
cg.isbn978-9934-9252-2-1en_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.alliancebiovciatAGRICULTUREen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 15 - Life on landen_US
dc.contributor.authorWilms, Hannesen_US
dc.contributor.authorPanis, Bartholomeusen_US
dc.date.accessioned2024-10-31T13:00:45Zen_US
dc.date.available2024-10-31T13:00:45Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/158333en_US
dc.titleThe impact of translocation mechanisms and plant architecture on the success or failure of propagation, a thidiazuron case studyen_US
dcterms.abstractWhile developing a propagation method for coconut (Cocos nucifera), we observed that the addition of thidiazuron (TDZ), a phenylurea based cytokinin, to the medium, did not elicit a reaction. We hypothesized that the apical dominance was too strong, preventing the TDZ from initiating shoot proliferation. To test this, we broke the apical dominance by cutting the plantlets vertically in two, removing the apical meristem from one piece. Both pieces were then placed in a medium containing TDZ. While this resulted in proliferation, more questions arose as it was the apical meristem that reacted. In other plants such as banana, this cut was not necessary, as there the axillary meristems on the outside of the explant proliferated. These observations lead us to hypothesize that TDZ might not be translocated through the plant the same way as adenine type cytokinins. To test this hypothesis, we placed a stem cutting of cactus fig containing many meristem along the stem, vertically in a medium containing BAP or TDZ. In the BAP medium this resulted in shoot formation of meristems on top of the plant, whilst in the case of the TDZ medium, the shoot formation originated from meristems submerged in the medium. With these observations, we can conclude that not all cytokinins are translocated in the same way. When developing proliferation protocols, both plant architecture and translocation of the PGR thus need to be taken into account, as these parameters can determine success or failureen_US
dcterms.accessRightsLimited Accessen_US
dcterms.bibliographicCitationWilms, H.; Panis, B. (2024) The impact of translocation mechanisms and plant architecture on the success or failure of propagation, a thidiazuron case study. Presented at the 2nd Conference of Cost Action CA21157 CopyTree In Vitro Culture of Wooden Crops: Problem Solving in New Approaches, 22-24 April, Jurmala (Latvia). Jūrmala (Latvia): Bulduri Technical School. p. 17. ISBN 978-9934-9252-2-1.en_US
dcterms.extent9 p.en_US
dcterms.issued2024-04-22en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.subjectmicropropagationen_US
dcterms.subjecttissue cultureen_US
dcterms.subjectcoconutsen_US
dcterms.typeAbstracten_US

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