Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationMaseno Universityen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationSwiss Tropical and Public Health Instituteen_US
cg.contributor.affiliationNelson Mandela Africa Institution of Science and Technologyen_US
cg.contributor.affiliationSokoine University of Agricultureen_US
cg.contributor.affiliationUniversity of Liverpoolen_US
cg.contributor.affiliationUniversity of Glasgowen_US
cg.contributor.affiliationCentre Suisse de Recherches Scientifiques en Côte d'Ivoireen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorDELTAS Africa Initiativeen_US
cg.contributor.donorMedical Research Council, United Kingdomen_US
cg.contributor.donorBiotechnology and Biological Sciences Research Council, United Kingdomen_US
cg.contributor.donorNatural Environment Research Council, United Kingdomen_US
cg.contributor.donorEconomic and Social Research Council, United Kingdomen_US
cg.contributor.donorAfrica Biosciences Challenge Funden_US
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen_US
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorGovernment of the United Kingdomen_US
cg.contributor.donorSwedish International Development Cooperation Agencyen_US
cg.coverage.countryKenyaen_US
cg.coverage.iso3166-alpha2KEen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierJames Akoko: 0000-0001-5730-4505en_US
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442en_US
cg.creator.identifierVelma Kivali: 0000-0003-0797-4484en_US
cg.creator.identifierEric M. Fèvre: 0000-0001-8931-4986en_US
cg.creator.identifierLaura Cristina Falzon: 0000-0002-4043-1644en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1186/s12917-020-02346-yen_US
cg.isijournalISI Journalen_US
cg.issn1746-6148en_US
cg.issue1en_US
cg.journalBMC Veterinary Researchen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriBRUCELLOSISen_US
cg.subject.ilriPIGSen_US
cg.volume16en_US
dc.contributor.authorAkoko, James M.en_US
dc.contributor.authorPelle, Rogeren_US
dc.contributor.authorKivali, Velmaen_US
dc.contributor.authorSchelling, E.en_US
dc.contributor.authorShirima, G.en_US
dc.contributor.authorMachuka, Eunice M.en_US
dc.contributor.authorMathew, C.en_US
dc.contributor.authorFèvre, Eric M.en_US
dc.contributor.authorKyallo, Victoriaen_US
dc.contributor.authorFalzon, Laura C.en_US
dc.contributor.authorLukambagire, A.S.en_US
dc.contributor.authorHalliday, J.E.B.en_US
dc.contributor.authorBonfoh, Bassirouen_US
dc.contributor.authorKazwala, R.en_US
dc.contributor.authorOuma, C.en_US
dc.date.accessioned2020-05-14T12:24:50Zen_US
dc.date.available2020-05-14T12:24:50Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/108242en_US
dc.titleSerological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenyaen_US
dcterms.abstractBackground Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2020-05-11en_US
dcterms.bibliographicCitationAkoko, J., Pelle, R., Kivali, V., Schelling, E., Shirima, G., Machuka, E.M., Mathew, C., Fèvre, E.M., Kyallo, V., Falzon, L.C., Lukambagire, A.S., Halliday, J.E.B., Bonfoh, B., Kazwala, R. and Ouma, C. 2020. Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya. BMC Veterinary Research 16: 133.en_US
dcterms.issued2020-12en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectbrucellaen_US
dcterms.subjectswineen_US
dcterms.subjectserologyen_US
dcterms.typeJournal Articleen_US

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