Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationMaseno Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationSwiss Tropical and Public Health Instituteen
cg.contributor.affiliationNelson Mandela Africa Institution of Science and Technologyen
cg.contributor.affiliationSokoine University of Agricultureen
cg.contributor.affiliationUniversity of Liverpoolen
cg.contributor.affiliationUniversity of Glasgowen
cg.contributor.affiliationCentre Suisse de Recherches Scientifiques en Côte d'Ivoireen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorDELTAS Africa Initiativeen
cg.contributor.donorMedical Research Council, United Kingdomen
cg.contributor.donorBiotechnology and Biological Sciences Research Council, United Kingdomen
cg.contributor.donorNatural Environment Research Council, United Kingdomen
cg.contributor.donorEconomic and Social Research Council, United Kingdomen
cg.contributor.donorAfrica Biosciences Challenge Funden
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorGovernment of the United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen
cg.creator.identifierJames Akoko: 0000-0001-5730-4505en
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442en
cg.creator.identifierVelma Kivali: 0000-0003-0797-4484en
cg.creator.identifierEric M. Fèvre: 0000-0001-8931-4986en
cg.creator.identifierLaura Cristina Falzon: 0000-0002-4043-1644en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1186/s12917-020-02346-yen
cg.isijournalISI Journalen
cg.issn1746-6148en
cg.issue1en
cg.journalBMC Veterinary Researchen
cg.reviewStatusPeer Reviewen
cg.subject.ilriBRUCELLOSISen
cg.subject.ilriPIGSen
cg.volume16en
dc.contributor.authorAkoko, James M.en
dc.contributor.authorPelle, Rogeren
dc.contributor.authorKivali, Velmaen
dc.contributor.authorSchelling, E.en
dc.contributor.authorShirima, G.en
dc.contributor.authorMachuka, Eunice M.en
dc.contributor.authorMathew, C.en
dc.contributor.authorFèvre, Eric M.en
dc.contributor.authorKyallo, Victoriaen
dc.contributor.authorFalzon, Laura C.en
dc.contributor.authorLukambagire, A.S.en
dc.contributor.authorHalliday, J.E.B.en
dc.contributor.authorBonfoh, Bassirouen
dc.contributor.authorKazwala, R.en
dc.contributor.authorOuma, C.en
dc.date.accessioned2020-05-14T12:24:50Zen
dc.date.available2020-05-14T12:24:50Zen
dc.identifier.urihttps://hdl.handle.net/10568/108242
dc.titleSerological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenyaen
dcterms.abstractBackground Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2020-05-11en
dcterms.bibliographicCitationAkoko, J., Pelle, R., Kivali, V., Schelling, E., Shirima, G., Machuka, E.M., Mathew, C., Fèvre, E.M., Kyallo, V., Falzon, L.C., Lukambagire, A.S., Halliday, J.E.B., Bonfoh, B., Kazwala, R. and Ouma, C. 2020. Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya. BMC Veterinary Research 16: 133.en
dcterms.issued2020-12en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectbrucellaen
dcterms.subjectswineen
dcterms.subjectserologyen
dcterms.typeJournal Article

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