Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid

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cg.contributor.affiliationBioversity International and the International Center for Tropical Agricultureen
cg.contributor.affiliationUniversity of Californiaen
cg.contributor.affiliationRoyal Holloway University of Londonen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryUnited States
cg.coverage.iso3166-alpha2US
cg.coverage.regionAmericas
cg.coverage.regionNorthern America
cg.coverage.regionLatin America and the Caribbean
cg.creator.identifierLuis Augusto Becerra Lopez-Lavalle: 0000-0003-3520-2270en
cg.creator.identifierAdriana Bohorquez-Chaux: 0000-0002-2430-5269en
cg.edition21:93en
cg.identifier.doihttps://doi.org/10.1186/s12864-019-6443-1en
cg.isijournalISI Journalen
cg.issn1471-2164en
cg.issue1en
cg.journalBMC Genomicsen
cg.reviewStatusPeer Reviewen
cg.volume21en
dc.contributor.authorIrigoyen, Maria L.en
dc.contributor.authorGarceau, Danielle Cen
dc.contributor.authorBohórquez Cháux, Adrianaen
dc.contributor.authorBecerra López Lavelle, Luis Augustoen
dc.contributor.authorPérez Fons, Lauraen
dc.contributor.authorFraser, Paul D.en
dc.contributor.authorWalling, Linda L.en
dc.date.accessioned2020-02-11T15:22:04Zen
dc.date.available2020-02-11T15:22:04Zen
dc.identifier.urihttps://hdl.handle.net/10568/107040
dc.titleGenome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic aciden
dcterms.abstractBackground: Whiteflies are a threat to cassava (Manihot esculenta), an important staple food in many tropical/ subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein families are an integral part of plant immunity. With the availability of whole genome sequences, the annotation and expression programs of the full complement of PR genes in an organism can now be achieved. An understanding of the responses of the entire complement of PR genes during biotic stress and to the defense hormones, salicylic acid (SA) and jasmonic acid (JA), is lacking. Here, we analyze the responses of cassava PR genes to whiteflies, SA, JA, and other biotic aggressors. Results: The cassava genome possesses 14 of the 17 plant PR families, with a total of 447 PR genes. A cassava PR gene nomenclature is proposed. Phylogenetic relatedness of cassava PR proteins to each other and to homologs in poplar, rice and Arabidopsis identified cassava-specific PR gene family expansions. The temporal programs of PR gene expression in response to the whitefly (Aleurotrachelus socialis) in four whitefly-susceptible cassava genotypes showed that 167 of the 447 PR genes were regulated after whitefly infestation. While the timing of PR gene expression varied, over 37% of whitefly-regulated PR genes were downregulated in all four genotypes. Notably, whitefly-responsive PR genes were largely coordinately regulated by SA and JA. The analysis of cassava PR gene expression in response to five other biotic stresses revealed a strong positive correlation between whitefly and Xanthomonas axonopodis and Cassava Brown Streak Virus responses and negative correlations between whitefly and Cassava Mosaic Virus responses. Finally, certain associations between PR genes in cassava expansions and response to biotic stresses were observed among PR families. Conclusions: This study represents the first genome-wide characterization of PR genes in cassava. PR gene responses to six biotic stresses and to SA and JA are demonstrably different to other angiosperms. We propose that our approach could be applied in other species to fully understand PR gene regulation by pathogens, pests and the canonical defense hormones SA and JA.en
dcterms.accessRightsOpen Access
dcterms.available2020-01-29en
dcterms.bibliographicCitationIrigoyen, Maria L.; Garceau, Danielle C.; Bohorquez-Chaux, Adriana; Becerra Lopez-Lavalle, Luis Augusto; Perez-Fons, Laura; Fraser, Paul D.; Walling, Linda L. (2020) Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid. BMC Genomics 21:93 18 p. ISSN: 1471-2164en
dcterms.extent1-18 pen
dcterms.issued2020-12en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectcassavaen
dcterms.subjectjasmonic aciden
dcterms.subjectpathogenesisen
dcterms.subjectanalysisen
dcterms.subjectmanihot esculentaen
dcterms.subjectmandiocaen
dcterms.subjectacido jasmonicoen
dcterms.subjectpatogenesisen
dcterms.typeJournal Article

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