First detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congo

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationPan African Universityen
cg.contributor.affiliationUniversité Evangélique en Afriqueen
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technologyen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.crpLivestock
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorGovernment of the United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.countryCongo, Democratic Republic of
cg.coverage.iso3166-alpha2CD
cg.coverage.regionAfrica
cg.coverage.regionMiddle Africa
cg.creator.identifierLucilla Steinaa: 0000-0003-3691-3971
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442
cg.creator.identifierChristian Keambou Tiambo: 0000-0002-7401-753X
cg.creator.identifierRoger Pelle: 0000-0003-1053-085X
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1186/s12985-020-01398-8en
cg.isijournalISI Journalen
cg.issn1743-422Xen
cg.issue1en
cg.journalVirology Journalen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriASFen
cg.subject.ilriPIGSen
cg.volume17en
dc.contributor.authorBisimwa, P.N.en
dc.contributor.authorOngus, J.R.en
dc.contributor.authorTiambo, Christian K.en
dc.contributor.authorMachuka, Eunice M.en
dc.contributor.authorBisimwa, E.B.en
dc.contributor.authorSteinaa, Lucillaen
dc.contributor.authorPelle, Rogeren
dc.date.accessioned2020-09-05T14:04:59Zen
dc.date.available2020-09-05T14:04:59Zen
dc.identifier.urihttps://hdl.handle.net/10568/109217
dc.titleFirst detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congoen
dcterms.abstractBackground African swine fever (ASF) is a highly contagious and severe hemorrhagic viral disease of domestic pigs. The analysis of variable regions of African swine fever virus (ASFV) genome led to more genotypic and serotypic information about circulating strains. The present study aimed at investigating the genetic diversity of ASFV strains in symptomatic pigs in South Kivu province of the Democratic Republic of Congo (DRC). Materials and methods Blood samples collected from 391 ASF symptomatic domestic pigs in 6 of 8 districts in South Kivu were screened for the presence of ASFV, using a VP73 gene-specific polymerase chain reaction (PCR) with the universal primer set PPA1-PPA2. To genotype the strains, we sequenced and compared the nucleotide sequences of PPA-positive samples at three loci: the C-terminus of B646L gene encoding the p72 protein, the E183L gene encoding the p54 protein, and the central hypervariable region (CVR) of the B602L gene encoding the J9L protein. In addition, to serotype and discriminate between closely related strains, the EP402L (CD2v) gene and the intergenic region between the I73R and I329L genes were analyzed. Results ASFV was confirmed in 26 of 391 pigs tested. However, only 19 and 15 PPA-positive samples, respectively, were successfully sequenced and phylogenetically analyzed for p72 (B646L) and p54 (E183L). All the ASFV studied were of genotype X. The CVR tetrameric repeat clustered the ASFV strains in two subgroups: the Uvira subgroup (10 TRS repeats, AAAABNAABA) and another subgroup from all other strains (8 TRS repeats, AABNAABA). The phylogenetic analysis of the EP402L gene clustered all the strains into CD2v serogroup 7. Analyzing the intergenic region between I73R and I329L genes revealed that the strains were identical but contained a deletion of a 33-nucleotide internal repeat sequence compared to ASFV strain Kenya 1950. Conclusion ASFV genotype X and serogroup 7 was identified in the ASF disease outbreaks in South Kivu province of DRC in 2018–2019. This represents the first report of ASFV genotype X in DRC. CVR tetrameric repeat sequences clustered the ASFV strains studied in two subgroups. Our finding emphasizes the need for improved coordination of the control of ASF.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2020-09-03
dcterms.bibliographicCitationBisimwa, P.N., Ongus, J.R., Tiambo, C.K., Machuka E.M., Bisimwa, E.B., Steinaa, L. and Pelle, R. 2020. First detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congo. Virology Journal 17: 135.en
dcterms.issued2020-12
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectafrican swine feveren
dcterms.subjectanimal diseasesen
dcterms.subjectswineen
dcterms.subjectvirusesen
dcterms.typeJournal Article

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