A 6.49-Mb inversion associated with the purple embryo spot trait in potato

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationChinese Academy of Agricultural Sciencesen
cg.contributor.affiliationYunnan Normal Universityen
cg.contributor.affiliationUniversity of Liègeen
cg.contributor.affiliationHuazhong Agricultural Universityen
cg.contributor.affiliationInternational Potato Centeren
cg.coverage.countryChina
cg.coverage.countryBelgium
cg.coverage.iso3166-alpha2CN
cg.coverage.iso3166-alpha2BE
cg.creator.identifierPhilip Kear: 0000-0002-4488-6013en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s42994-025-00197-5en
cg.isijournalISI Journalen
cg.issn2731-7601en
cg.issue1en
cg.journalaBIOTECHen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.cipPOTATOESen
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCIen
cg.subject.cipCROP PROTECTIONen
cg.subject.cipBREEDINGen
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.subject.sdgSDG 9 - Industry, innovation and infrastructureen
cg.subject.sdgSDG 13 - Climate actionen
cg.volume6en
dc.contributor.authorWang, P.en
dc.contributor.authorCheng, L.en
dc.contributor.authorPan, J.en
dc.contributor.authorMa, L.en
dc.contributor.authorHu, X.en
dc.contributor.authorZhang, Z.en
dc.contributor.authorLi, D.en
dc.contributor.authorZhu, Y.en
dc.contributor.authorChang, S.en
dc.contributor.authorYuan, P.en
dc.contributor.authorKear, P.en
dc.contributor.authorLassois, L.en
dc.contributor.authorZhu, G.en
dc.contributor.authorHuang, S.en
dc.contributor.authorDu, H.en
dc.contributor.authorZhang, C.en
dc.date.accessioned2025-03-28T14:49:37Zen
dc.date.available2025-03-28T14:49:37Zen
dc.identifier.urihttps://hdl.handle.net/10568/173925
dc.titleA 6.49-Mb inversion associated with the purple embryo spot trait in potatoen
dcterms.abstractThe embryo spot trait leads to a deep purple or reddish coloration at the base of the cotyledons of the embryo, visible on both sides of flat potato (Solanum tuberosum) seeds. This trait has long been used by potato researchers and breeders as a morphological marker during dihaploid induction. The formation of embryo spots reflects the accumulation of anthocyanins, but the genetic basis of this trait remains unclear. In this study, we mapped the embryo spot trait to a 6.78-Mb region at the end of chromosome 10 using an F2 population derived from a cross between spotted and spotless plants. The recombination rate in the candidate region is severely suppressed, posing challenges for the map-based cloning of the underlying gene and suggesting large-scale rearrangements in this region. A de novo genome assembly of the spotted individual and a comparative genomic analysis to the reference genome of spotless potato revealed a 6.49-Mb inversion present in the spotted plant genome. The left breakpoint of this inversion occurred in the promoter region of an R2R3 MYB transcription factor gene that is highly expressed in the cotyledon base of spotted embryos but is not expressed in that of spotless embryos. This study elucidated the genetic basis for embryo spot formation in potato and provides a foundation for future cloning of the causative gene.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.audienceFarmersen
dcterms.audienceGeneral Publicen
dcterms.audienceNGOsen
dcterms.audiencePolicy Makersen
dcterms.audienceScientistsen
dcterms.bibliographicCitationWang, P.; Cheng, L.; Pan, J.; Ma, L.; Hu, X.; Zhang, Z.; Li, D.; Zhu, Y.; Chang, S.; Yuan, P.; Kear, P.; Lassois, L.; Zhu, G.; Huang, S.; Du, H.; Zhang, C. 2025. A 6.49-Mb inversion associated with the purple embryo spot trait in potato. aBIOTECH. ISSN 2662-1738. 6(1), 22–32. https://doi.org/10.1007/s42994-025-00197-5en
dcterms.extent22–32en
dcterms.issued2025-01-18en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.subjectpotatoesen
dcterms.subjectgeneticsen
dcterms.subjectanthocyaninsen
dcterms.typeJournal Article

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