Genome analysis and pathobiology of Cassava-Infecting Torradoviruses containing a putative Maf/HAM1 pyrophosphatase domain

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.affiliationUniversidad Nacional de Colombiaen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.contributor.initiativeGenebanks
cg.creator.identifierMonica Carvajal-Yepes: 0000-0003-2709-7909en
cg.creator.identifierJenyfer Jimenez: 0000-0001-8149-6615en
cg.creator.identifierJohn Eiver Belalcazar Martinez: 0000-0002-4418-2526en
cg.creator.identifierWilmer J. Cuellar: 0000-0003-4702-3237en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1094/pdis-11-21-2520-reen
cg.isijournalISI Journalen
cg.issn1943-7692en
cg.issue11en
cg.journalPlant Diseaseen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.alliancebiovciatCASSAVAen
cg.subject.alliancebiovciatPESTS AND DISEASESen
cg.subject.impactAreaPoverty reduction, livelihoods and jobs
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume106(11)en
dc.contributor.authorCarvajal-Yepes, Mónicaen
dc.contributor.authorJiménez Polo, Jenyferen
dc.contributor.authorBelalcázar, John Eiveren
dc.contributor.authorCuásquer, Juan B.en
dc.contributor.authorLozano, Ivanen
dc.contributor.authorOlaya, Cristian A.en
dc.contributor.authorCuéllar, Wilmer Joseen
dc.date.accessioned2022-11-30T14:42:01Zen
dc.date.available2022-11-30T14:42:01Zen
dc.identifier.urihttps://hdl.handle.net/10568/125732
dc.titleGenome analysis and pathobiology of Cassava-Infecting Torradoviruses containing a putative Maf/HAM1 pyrophosphatase domainen
dcterms.abstractNext generation sequencing has been used to identify and characterize the full genome sequence of a cassava-infecting torradovirus, revealing the presence of a Maf/HAM1 domain downstream of the RNA-dependent RNA-polymerase (RdRp) domain in RNA1 in all isolates sequenced. A similar domain is also found in unrelated potyvirids infecting Euphorbiaceae hosts in the Americas and cassava in Africa. Even though cassava torrado-like virus (CsTLV) could not be mechanically transmitted to a series of herbaceous hosts, it can be efficiently transmitted by bud graft-inoculation to different cassava landraces. Our bioassays show that CsTLV has a narrow host range. Crystal-like structures of isometric virus-like particles were observed in cells of plants with single infection by CsTLV, and consistently induced chlorotic leaf spots and affected root yields significantly. Moreover, CsTLV infection induces changes in the accumulation of total sugars in storage roots. Field surveys indicated the presence of CsTLV in the main cassava growing regions of Colombia, and the occurrence of two different cassava-infecting torradovirus species. Profiles of small RNAs of 21 to 24 nucleotides in length, derived from CsTLV RNAs targeted by cassava RNA silencing defense mechanisms, are also reported.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationCarvajal-Yepes, M.: Jimenez, J.; Belalcazar, J.; Cuasquer, J.B.: Lozano, I.: Olaya, C.A.: Cuellar, W.J. (2022) Genome analysis and pathobiology of Cassava-infecting torradoviruses containing a putative Maf/HAM1 pyrophosphatase domain. Plant Disease 106(11) p. 2808-2816. ISSN: 1943-7692en
dcterms.extent2808-2816en
dcterms.issued2022-11-01en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherScientific Societiesen
dcterms.subjectmanihot esculentaen
dcterms.subjectgenomesen
dcterms.subjectdna sequencesen
dcterms.subjectcarbohydrate contenten
dcterms.subjectrootsen
dcterms.subjectyieldsen
dcterms.subjectplant virusesen
dcterms.subjectgenomasen
dcterms.subjectsecuencia de adnen
dcterms.typeJournal Article

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