Genome analysis and pathobiology of Cassava-Infecting Torradoviruses containing a putative Maf/HAM1 pyrophosphatase domain

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Center for Tropical Agricultureen_US
cg.contributor.affiliationUniversidad Nacional de Colombiaen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen_US
cg.contributor.initiativeGenebanksen_US
cg.creator.identifierMonica Carvajal-Yepes: 0000-0003-2709-7909en_US
cg.creator.identifierJenyfer Jimenez: 0000-0001-8149-6615en_US
cg.creator.identifierJohn Eiver Belalcazar Martinez: 0000-0002-4418-2526en_US
cg.creator.identifierWilmer J. Cuellar: 0000-0003-4702-3237en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1094/pdis-11-21-2520-reen_US
cg.isijournalISI Journalen_US
cg.issn1943-7692en_US
cg.issue11en_US
cg.journalPlant Diseaseen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.alliancebiovciatCASSAVAen_US
cg.subject.alliancebiovciatPESTS AND DISEASESen_US
cg.subject.impactAreaPoverty reduction, livelihoods and jobsen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.volume106(11)en_US
dc.contributor.authorCarvajal-Yepes, Mónicaen_US
dc.contributor.authorJiménez Polo, Jenyferen_US
dc.contributor.authorBelalcázar, John Eiveren_US
dc.contributor.authorCuásquer, Juan B.en_US
dc.contributor.authorLozano, Ivanen_US
dc.contributor.authorOlaya, Cristian A.en_US
dc.contributor.authorCuéllar, Wilmer Joseen_US
dc.date.accessioned2022-11-30T14:42:01Zen_US
dc.date.available2022-11-30T14:42:01Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/125732en_US
dc.titleGenome analysis and pathobiology of Cassava-Infecting Torradoviruses containing a putative Maf/HAM1 pyrophosphatase domainen_US
dcterms.abstractNext generation sequencing has been used to identify and characterize the full genome sequence of a cassava-infecting torradovirus, revealing the presence of a Maf/HAM1 domain downstream of the RNA-dependent RNA-polymerase (RdRp) domain in RNA1 in all isolates sequenced. A similar domain is also found in unrelated potyvirids infecting Euphorbiaceae hosts in the Americas and cassava in Africa. Even though cassava torrado-like virus (CsTLV) could not be mechanically transmitted to a series of herbaceous hosts, it can be efficiently transmitted by bud graft-inoculation to different cassava landraces. Our bioassays show that CsTLV has a narrow host range. Crystal-like structures of isometric virus-like particles were observed in cells of plants with single infection by CsTLV, and consistently induced chlorotic leaf spots and affected root yields significantly. Moreover, CsTLV infection induces changes in the accumulation of total sugars in storage roots. Field surveys indicated the presence of CsTLV in the main cassava growing regions of Colombia, and the occurrence of two different cassava-infecting torradovirus species. Profiles of small RNAs of 21 to 24 nucleotides in length, derived from CsTLV RNAs targeted by cassava RNA silencing defense mechanisms, are also reported.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.bibliographicCitationCarvajal-Yepes, M.: Jimenez, J.; Belalcazar, J.; Cuasquer, J.B.: Lozano, I.: Olaya, C.A.: Cuellar, W.J. (2022) Genome analysis and pathobiology of Cassava-infecting torradoviruses containing a putative Maf/HAM1 pyrophosphatase domain. Plant Disease 106(11) p. 2808-2816. ISSN: 1943-7692en_US
dcterms.extent2808-2816en_US
dcterms.issued2022-11-01en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherScientific Societiesen_US
dcterms.subjectmanihot esculentaen_US
dcterms.subjectgenomesen_US
dcterms.subjectdna sequencesen_US
dcterms.subjectcarbohydrate contenten_US
dcterms.subjectrootsen_US
dcterms.subjectyieldsen_US
dcterms.subjectplant virusesen_US
dcterms.subjectgenomasen_US
dcterms.subjectsecuencia de adnen_US
dcterms.typeJournal Articleen_US

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