Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationWageningen University & Researchen
cg.contributor.crpLivestock
cg.contributor.donorNorman Borlaug Commemorative Research Initiativeen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorUnited States Agency for International Developmenten
cg.creator.identifierSonal P Henson: 0000-0002-2002-5462
cg.creator.identifierLucilla Steinaa: 0000-0003-3691-3971
cg.creator.identifierRoger Pelle: 0000-0003-1053-085X
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.vetimm.2018.03.004en
cg.isijournalISI Journalen
cg.issn0165-2427en
cg.journalVeterinary Immunology and Immunopathologyen
cg.reviewStatusPeer Reviewen
cg.speciesTheileria parvaen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriCATTLEen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriECFen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriVACCINESen
cg.volume199en
dc.contributor.authorNyagwange, Jamesen
dc.contributor.authorNene, Vishvanath M.en
dc.contributor.authorMwalimu, Stephenen
dc.contributor.authorHenson, Sonal P.en
dc.contributor.authorSteinaa, Lucillaen
dc.contributor.authorNzau, Benjamin M.en
dc.contributor.authorTijhaar, Edwinen
dc.contributor.authorPelle, Rogeren
dc.date.accessioned2018-05-11T14:04:28Zen
dc.date.available2018-05-11T14:04:28Zen
dc.identifier.urihttps://hdl.handle.net/10568/92522
dc.titleAntibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitroen
dcterms.abstractEast Coast fever (ECF) caused by Theileria parva kills cattle in East, Central and Southern Africa leading to significant economic losses. Vaccination is used as a control strategy against ECF and is presently dependent on deliberate infection with live sporozoites and simultaneous treatment with a long-acting oxytetracycline. Although effective, this method has serious limitations; the immunity is parasite strain specific and immunized cattle can become life-long asymptomatic carriers of the parasite, posing risk for the spread of the disease. In efforts to develop a subunit vaccine, the role of antibodies in the neutralization of T. parva sporozoites infection of host cells has been investigated and a circumsporozoite protein, p67, is able to induce such neutralizing antibodies. However, the p67 protein only protects a proportion of immunized cattle against T. parva challenge and such protection might be improved by inclusion of additional parasite antigens that neutralize sporozoite infection. In an attempt to identify such antigens, we searched the re-annotated T. parva genome for genes predicted to contain GPI anchor signals, since they are likely to be located on the cell surface, and expressed fragments of six of the selected genes in E. coli. The recombinant proteins were used to raise antisera in mice. Antisera to two proteins, TpMuguga_01g00876 and TpMuguga_01g00939, neutralized sporozoite infectivity to a high degree, while antisera to two additional proteins, TpMuguga_01g00095 and TpMuguga_04g00437, exhibited moderate neutralizing capacity. We conclude that these four antigens are potential vaccine candidates, which should be evaluated further in cattle.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationNyagwange, J., Nene, V., Mwalimu, S., Henson, S., Steinaa, L., Nzau, B., Tijhaar, E. and Pelle, R. 2018. Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro. Veterinary Immunology and Immunopathology 199:8-14.en
dcterms.extentp. 8-14en
dcterms.issued2018-05
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherElsevieren
dcterms.subjecttheileriaen
dcterms.subjectcattleen
dcterms.subjectanimal diseasesen
dcterms.subjectvaccinesen
dcterms.subjectdisease controlen
dcterms.subjecteast coast feveren
dcterms.typeJournal Article

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Improved vaccines for the control of East Coast fever in cattle in Africa