Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro

Simple item page
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationWageningen University & Researchen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorNorman Borlaug Commemorative Research Initiativeen_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorUnited States Agency for International Developmenten_US
cg.creator.identifierSonal P Henson: 0000-0002-2002-5462en_US
cg.creator.identifierLucilla Steinaa: 0000-0003-3691-3971en_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.vetimm.2018.03.004en_US
cg.isijournalISI Journalen_US
cg.issn0165-2427en_US
cg.journalVeterinary Immunology and Immunopathologyen_US
cg.reviewStatusPeer Reviewen_US
cg.speciesTheileria parvaen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriVACCINESen_US
cg.volume199en_US
dc.contributor.authorNyagwange, Jamesen_US
dc.contributor.authorNene, Vishvanath M.en_US
dc.contributor.authorMwalimu, Stephenen_US
dc.contributor.authorHenson, Sonal P.en_US
dc.contributor.authorSteinaa, Lucillaen_US
dc.contributor.authorNzau, Benjamin M.en_US
dc.contributor.authorTijhaar, Edwinen_US
dc.contributor.authorPelle, Rogeren_US
dc.date.accessioned2018-05-11T14:04:28Zen_US
dc.date.available2018-05-11T14:04:28Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/92522en_US
dc.titleAntibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitroen_US
dcterms.abstractEast Coast fever (ECF) caused by Theileria parva kills cattle in East, Central and Southern Africa leading to significant economic losses. Vaccination is used as a control strategy against ECF and is presently dependent on deliberate infection with live sporozoites and simultaneous treatment with a long-acting oxytetracycline. Although effective, this method has serious limitations; the immunity is parasite strain specific and immunized cattle can become life-long asymptomatic carriers of the parasite, posing risk for the spread of the disease. In efforts to develop a subunit vaccine, the role of antibodies in the neutralization of T. parva sporozoites infection of host cells has been investigated and a circumsporozoite protein, p67, is able to induce such neutralizing antibodies. However, the p67 protein only protects a proportion of immunized cattle against T. parva challenge and such protection might be improved by inclusion of additional parasite antigens that neutralize sporozoite infection. In an attempt to identify such antigens, we searched the re-annotated T. parva genome for genes predicted to contain GPI anchor signals, since they are likely to be located on the cell surface, and expressed fragments of six of the selected genes in E. coli. The recombinant proteins were used to raise antisera in mice. Antisera to two proteins, TpMuguga_01g00876 and TpMuguga_01g00939, neutralized sporozoite infectivity to a high degree, while antisera to two additional proteins, TpMuguga_01g00095 and TpMuguga_04g00437, exhibited moderate neutralizing capacity. We conclude that these four antigens are potential vaccine candidates, which should be evaluated further in cattle.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationNyagwange, J., Nene, V., Mwalimu, S., Henson, S., Steinaa, L., Nzau, B., Tijhaar, E. and Pelle, R. 2018. Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro. Veterinary Immunology and Immunopathology 199:8-14.en_US
dcterms.extentp. 8-14en_US
dcterms.issued2018-05en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherElsevieren_US
dcterms.subjecttheileriaen_US
dcterms.subjectcattleen_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectvaccinesen_US
dcterms.subjectdisease controlen_US
dcterms.subjecteast coast feveren_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
CRP Livestock journal articles
ILRI animal and human health program outputs (2017-2024)
ILRI BecA articles
Improved vaccines for the control of East Coast fever in cattle in Africa