Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.affiliationRwanda Agriculture Boarden
cg.contributor.affiliationInstitut des Sciences Agronomiques du Burundien
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryBurundi
cg.coverage.iso3166-alpha2BI
cg.coverage.regionAfrica
cg.coverage.regionSub-Saharan Africa
cg.coverage.regionEastern Africa
cg.creator.identifierJoshua Okonya: 0000-0002-9874-5021
cg.creator.identifierHeidy Gamarra: 0000-0003-2746-2849
cg.creator.identifierAstere BARARYENYA: 0000-0002-1724-0006
cg.creator.identifierJürgen Kroschel: 0000-0002-7064-6179
cg.creator.identifierJan Kreuze: 0000-0002-6116-9200
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.virusres.2021.198487en
cg.isijournalISI Journalen
cg.issn1872-7492en
cg.journalVirus Researchen
cg.reviewStatusPeer Reviewen
cg.subject.cipCROP AND SYSTEMS SCIENCES CSSen
cg.subject.cipPOTATO AGRI-FOOD SYSTEMSen
cg.subject.cipPOTATOESen
cg.volume302en
dc.contributor.authorOkonya, J.en
dc.contributor.authorGamarra, H.en
dc.contributor.authorNduwayezu, A.en
dc.contributor.authorBararyenya, A.en
dc.contributor.authorKroschel, Jürgenen
dc.contributor.authorKreuze, Jan F.en
dc.date.accessioned2021-09-10T05:33:42Zen
dc.date.available2021-09-10T05:33:42Zen
dc.identifier.urihttps://hdl.handle.net/10568/114945
dc.titleSerological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africaen
dcterms.abstractWorldwide, potato (Solanum tuberosum L.) is the third most important food crop after rice and wheat. Its production is however constrained by several virus diseases. The occurrence and distribution of the economically important viruses and associated insect vectors is however not known for Rwanda and Burundi, where potato is an important food security and income crop. We surveyed 194 potato fields for viruses and insect vectors. Aphids were commonly found infesting farmers' potato fields in contrast to whiteflies. Testing by Enzyme Linked Immunosorbent Assay (ELISA) for six potato viruses identified five viruses: potato leafroll virus (PLRV), potato virus X, S, M and Y (PVX, PVS, PVM, PVY) in Rwanda and two viruses (PLRV and PVS) in Burundi. A subset of samples were analyzed using small RNA sequencing and assembly (sRSA) and additionally revealed presence of PVX and for the first time, tobacco rattle virus (TRV) in Burundi. PLRV and PVS were most common while PVY was rare and not found in Burundi, which is highly unusual. To our knowledge, this is the first report of TRV infecting potatoes in sub-Saharan Africa. Phylogenetic analysis of 14 complete viral genomes determined by sRSA suggested multiple introductions of viruses into the region.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.bibliographicCitationOkonya, J. S., Gamarra, H., Nduwayezu, A., Bararyenya, A., Kroschel, J., & Kreuze, J. (2021). Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa. Virus Research. ISSN 1872-7492. 302, 198487en
dcterms.issued2021-09
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectsolanum tuberosumen
dcterms.subjectaphidoideaen
dcterms.subjectvirosesen
dcterms.subjectpotatoesen
dcterms.typeJournal Article

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