A real-time fluorescent reverse transcription quantitative PCR assay for rapid detection of genetic markers’ expression associated with Fusarium Wilt of banana biocontrol activities in bacillus

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationYunnan Academy of Agricultural Sciences, Chinaen
cg.contributor.affiliationGuangxi Academy of Agricultural Sciences, Chinaen
cg.contributor.affiliationYunnan Agricultural Universityen
cg.contributor.affiliationBioversity Internationalen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorYunnan Provincial Government, Chinaen
cg.creator.identifierSi-Jun Zheng: 0000-0003-1550-3738
cg.identifier.doihttps://doi.org/10.3390/jof7050353en
cg.isijournalISI Journalen
cg.issn2309-608Xen
cg.issue5en
cg.journalJournal of Fungien
cg.reviewStatusPeer Reviewen
cg.subject.alliancebiovciatBIODIVERSITYen
cg.subject.impactAreaEnvironmental health and biodiversity
cg.subject.sdgSDG 15 - Life on landen
cg.volume7en
dc.contributor.authorLi, Shuen
dc.contributor.authorHe, Pingen
dc.contributor.authorFan, Huacaien
dc.contributor.authorLiu, Linaen
dc.contributor.authorYin, Kesuoen
dc.contributor.authorYang, Baomingen
dc.contributor.authorLi, Yongpingen
dc.contributor.authorHuang, Su-Meien
dc.contributor.authorLi, Xundongen
dc.contributor.authorZheng, Si-Junen
dc.date.accessioned2021-05-21T10:04:41Zen
dc.date.available2021-05-21T10:04:41Zen
dc.identifier.urihttps://hdl.handle.net/10568/113757
dc.titleA real-time fluorescent reverse transcription quantitative PCR assay for rapid detection of genetic markers’ expression associated with Fusarium Wilt of banana biocontrol activities in bacillusen
dcterms.abstractFusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), especially Tropical Race 4 (TR4), seriously threatens banana production worldwide. There is no single effective control measure, although certain Bacillus strains secrete antibiotics as promising disease-biocontrol agents. This study identified five Bacillus strains displaying strong antibiotic activity against TR4, using a systemic assessment for presence/absence of genetic markers at genome level, and expres sion profiles at transcriptome level. A conventional PCR with 13 specific primer pairs detected biocontrol-related genes. An accurate, quantitative real-time PCR protocol with novel designed specific primers was developed to characterise strain-specific gene expression, that optimises strain culturing and RNA-isolation methodologies. Six genes responsible for synthesising non-ribosomal peptide synthetase biocontrol metabolites were detected in all five strains. Three genes were involved in synthesising three Polyketide synthetase metabolites in all five strains, but the macrolactin synthase gene mln was only detected in WBN06 and YN1282-2. All five Bacillus strains have the genes dhb and bioA, essential for synthesising bacillibactin and biotin. However, the gene sboA, involved in subtilisin synthesis, is absent in all five strains. These genes’ expression patterns were significantly different among these strains, suggesting different mechanisms involved in TR4 biocontrol. Results will help elucidate functional genes’ biocontrol mechanisms.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2021-04-30
dcterms.bibliographicCitationLi, S.; He, P.; Fan, H.; Liu, L.; Yin, K.; Yang, B.; Li, Y.; Huang, S.-M.; Li, X.; Zheng, S.-J. (2021) A real-time fluorescent reverse transcription quantitative PCR assay for rapid detection of genetic markers’ expression associated with Fusarium Wilt of banana biocontrol activities in bacillus. Journal of Fungi 7(5): 353. ISSN: 2309-608Xen
dcterms.issued2021-04-30
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherMDPIen
dcterms.subjectbacillusen
dcterms.subjectbiological controlen
dcterms.subjectgenetic markersen
dcterms.subjecttranscription factorsen
dcterms.subjectfusarium wilten
dcterms.subjectplant disease controlen
dcterms.subjectcontrol biológicoen
dcterms.subjectmarcadores genéticosen
dcterms.subjectfactores de transcripciónen
dcterms.typeJournal Article

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