Detection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisia

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversité de la Manoubaen
cg.contributor.affiliationOffice de l'Elevage et des Pâturage, Tunisiaen
cg.contributor.affiliationInstitut National de la Recherche Agronomique du Nigeren
cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.crpLivestocken
cg.contributor.crpDryland Systemsen
cg.contributor.donorMinistère de l'Enseignement Supérieur et de la Recherche Scientifique, Tunisiaen
cg.coverage.countryTunisiaen
cg.coverage.iso3166-alpha2TNen
cg.coverage.regionAfricaen
cg.coverage.regionNorthern Africaen
cg.creator.identifierMourad Rekik: 0000-0001-7455-2017en
cg.creator.identifierBarbara Wieland: 0000-0003-4020-9186en
cg.creator.identifierJoram Mwacharo: 0000-0001-6981-8140en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.vprsr.2019.100341en
cg.isijournalISI Journalen
cg.issn2405-9390en
cg.journalVeterinary Parasitologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriANIMAL HEALTHen
cg.subject.ilriRESEARCHen
cg.subject.ilriSHEEPen
cg.volume18en
dc.contributor.authorRouatbi, M.en
dc.contributor.authorAmairia, S.en
dc.contributor.authorLahmer, M.en
dc.contributor.authorLassoued, N.en
dc.contributor.authorRekik, Mouraden
dc.contributor.authorWieland, Barbaraen
dc.contributor.authorMwacharo, Joram M.en
dc.contributor.authorGharbi, M.en
dc.date.accessioned2019-12-02T13:21:11Zen
dc.date.available2019-12-02T13:21:11Zen
dc.identifier.urihttps://hdl.handle.net/10568/105979
dc.titleDetection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisiaen
dcterms.abstractToxoplasmosis, a parasitic infection, disrupts sexual functions resulting in decline in reproductive and economic performance in sheep. Lacking discernible symptoms, toxoplasmosis is difficult to detect and diagnose in infected animals. Here, to estimate the prevalence of natural infection by Toxoplasma gondii, we used PCR to amplify and detect T. gondii DNA in semen from 92 rams of three breeds from four regions in Tunisia and seroprevalence was determined using a commercial ELISA kit. From the PCR amplifications, 51.09 ± 10.21% of the rams were tested positive for T. gondii with an overall seroprevalence of 39.13 ± 9.97%. Risk factors including ram location and number of accomplished mating seasons significantly (p < .05) affected the sero- and molecular prevalence of T. gondii in semen but, there was a fair concordance between sero- and molecular prevalence (Kappa = 0.33). Sequences of T. gondii from five positive samples were 100% identical (same haplotype). Comparison of these sequences with those archived at the GenBank showed a sequence similarity range between 95 and 100%. The haplotype defining the five Tunisian sequences was similar to the one observed in chicken, cats, European pole cat and humans from Brazil, St Kitts and Nevis, Great Britain and Tunisia, respectively. This indicates its wide geographic distribution and non-species specificity. Our findings suggest a high prevalence of toxoplasmosis in Tunisian matting rams; further studies concerning its venereal transmission capacity are needed prior to recommending a systematic screening of T. gondii DNA in rams' semen used for both natural breeding and artificial insemination.en
dcterms.accessRightsLimited Accessen
dcterms.audienceScientistsen
dcterms.bibliographicCitationRouatbi, M., Amairia, S., Lahmer, M., Lassoued, N., Rekik, M., Wieland, B., Mwacharo, J.M. and Gharbi, M. 2019. Detection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisia. Veterinary Parasitology: Regional Studies and Reports 18: 100341.en
dcterms.issued2019-12en
dcterms.languageenen
dcterms.licenseCopyrighted; all rights reserveden
dcterms.publisherElsevieren
dcterms.subjectcommercial farmingen
dcterms.subjectsheepen
dcterms.subjectinfectionen
dcterms.subjectanimal diseasesen
dcterms.subjectanimal healthen
dcterms.subjectparasitologyen
dcterms.typeJournal Articleen

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