Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda

cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationFederal Institute for Risk Assessment, Germanyen
cg.contributor.affiliationMakerere Universityen
cg.contributor.affiliationInstitute of Virology and Immunology, Switzerlanden
cg.contributor.affiliationUniversity of Bernen
cg.contributor.affiliationUniversity of Copenhagenen
cg.contributor.affiliationNational Agricultural Research Organization, Ugandaen
cg.contributor.affiliationUniversity of Liverpoolen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpLivestock
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierDICKSON NDOBOLI: 0000-0003-4764-5861
cg.creator.identifierDishon Muloi: 0000-0002-6236-2280
cg.creator.identifierKristina Roesel: 0000-0002-2553-1129
cg.creator.identifierMichel Dione: 0000-0001-7812-5776
cg.creator.identifierArshnee Moodley: 0000-0002-6469-3948
cg.creator.identifierBarbara Wieland: 0000-0003-4020-9186
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.onehlt.2024.100762en
cg.isijournalISI Journalen
cg.issn2352-7714en
cg.journalOne Healthen
cg.reviewStatusPeer Reviewen
cg.speciesEscherichia colien
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.ilriAMRen
cg.subject.ilriPOULTRYen
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactPlatformNutrition, Health and Food Security
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.volume18en
dc.contributor.authorMbatidde, Ireneen
dc.contributor.authorNdoboli, Dicksonen
dc.contributor.authorAyebare, Drecken
dc.contributor.authorMuloi, Dishonen
dc.contributor.authorRoesel, Kristinaen
dc.contributor.authorOchieng, Linneten
dc.contributor.authorDione, Michel M.en
dc.contributor.authorTenhagen, B.-A.en
dc.contributor.authorBiryomumaisho, S.en
dc.contributor.authorWampande, E.en
dc.contributor.authorWieland, Barbaraen
dc.contributor.authorOlsen, J.E.en
dc.contributor.authorMoodley, Arshneeen
dc.date.accessioned2024-05-24T12:09:52Zen
dc.date.available2024-05-24T12:09:52Zen
dc.identifier.urihttps://hdl.handle.net/10568/144045
dc.titleAntimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Ugandaen
dcterms.abstractLivestock associated antimicrobial resistance (AMR) can reduce productivity and cause economic losses, threatening the livelihoods of poor farming communities in low-income settings. We investigated the practices and risk factors for increased antibiotic use, and AMR in <i>Escherichia coli</i> including resistance to human critically important antibiotics like cefotaxime and colistin in semi-intensive and free-range poultry farms in Uganda. Samples and farm management data were collected from 402 poultry farms in two districts between October 2021 to March 2022. Samples were processed to isolate <i>E. coli</i> and to quantify cefotaxime (CTX) and colistin (COL) resistant coliforms. The identification of presumptive <i>E. coli</i> isolated on MacConkey agar without antibiotics, was confirmed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and subjected to antimicrobial susceptibility testing by disk diffusion using EUCAST guidelines. Our models indicated that antibiotic use was associated with production intensity, and type of feed used. Moreover, semi-intensive farmers had better knowledge on antibiotic use compared to farmers in the free-range system. In semi-intensive farms, 52% harbored COL<sup>R</sup> and 57% CTX<sup>R</sup> coliforms. In free-range farms, 54% had COL<sup>R</sup> and 67% CTX<sup>R</sup> coliforms. Resistance to tetracycline, ampicillin and enrofloxacin were more frequent in semi-intensive farms compared to the free-range farms. Multi-drug resistant <i>E. coli</i> were identified in both poultry production systems despite different management and antibiotic use practices. There was no significant relationship between antibiotic use and resistance for the six antibiotics tested.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2024-05-23
dcterms.bibliographicCitationMbatidde, I., Ndoboli, D., Ayebare, D., Muloi, D., Roesel, K., Ochieng, L., Dione, M., Tenhagen, B.-A., Biryomumaisho, S., Wampande, E., Wieland, B., Olsen, J.E. and Moodley, A. 2024. Antimicrobial use and antimicrobial resistance in <i>Escherichia coli</i> in semi-intensive and free-range poultry farms in Uganda. <i>One Health</i> 18: 100762.en
dcterms.extent100762en
dcterms.issued2024-06
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherElsevieren
dcterms.subjectantimicrobial resistanceen
dcterms.subjectpoultryen
dcterms.typeJournal Article

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