Non-typhoidal Salmonella among slaughterhouse workers and in the pork value chain in selected districts of Uganda

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationFreie Universität Berlinen_US
cg.contributor.affiliationUniversity of Prince Edward Islanden_US
cg.contributor.affiliationUniversity of Bernen_US
cg.contributor.affiliationMinistry of Agriculture, Animal Industry and Fisheries, Ugandaen_US
cg.contributor.affiliationMakerere Universityen_US
cg.contributor.affiliationFederal Institute for Risk Assessment, Germanyen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeOne Healthen_US
cg.coverage.countryUgandaen_US
cg.coverage.iso3166-alpha2UGen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierVelma Kivali: 0000-0003-0797-4484en_US
cg.creator.identifierKristina Roesel: 0000-0002-2553-1129en_US
cg.creator.identifierLordrick Alinaitwe: 0000-0002-5432-6407en_US
cg.creator.identifierJames Bugeza: 0000-0002-8124-2807en_US
cg.creator.identifierSinh Dang-Xuan: 0000-0002-0522-7808en_US
cg.creator.identifierElizabeth Cook: 0000-0001-6081-8363en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.3389/fvets.2024.1427773en_US
cg.isijournalISI Journalen_US
cg.issn2297-1769en_US
cg.journalFrontiers in Veterinary Scienceen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.ilriANIMAL PRODUCTSen_US
cg.subject.ilriFOOD SAFETYen_US
cg.subject.ilriHUMAN HEALTHen_US
cg.subject.ilriPIGSen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.impactPlatformNutrition, Health and Food Securityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.volume11en_US
dc.contributor.authorKivali, Velmaen_US
dc.contributor.authorRoesel, Kristinaen_US
dc.contributor.authorDohoo, I.en_US
dc.contributor.authorAlinaitwe, Lordricken_US
dc.contributor.authorBugeza, James K.en_US
dc.contributor.authorHoona, J.J.en_US
dc.contributor.authorMugizi, Denis R.en_US
dc.contributor.authorKankya, C.en_US
dc.contributor.authorSinh Dang-Xuanen_US
dc.contributor.authorSzabo, I.en_US
dc.contributor.authorRösler, U.en_US
dc.contributor.authorFriese, A.en_US
dc.contributor.authorCook, Elizabeth A.J.en_US
dc.date.accessioned2024-09-17T16:20:07Zen_US
dc.date.available2024-09-17T16:20:07Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/152271en_US
dc.titleNon-typhoidal Salmonella among slaughterhouse workers and in the pork value chain in selected districts of Ugandaen_US
dcterms.abstractIntroduction: Non-typhoidal <i>Salmonella</i> (NTS) is a major cause of gastroenteritis worldwide, often associated with meat consumption and meat processing. Research on NTS infection and circulating serovars in meat value chains in Uganda is limited. We aimed to establish NTS prevalence, antimicrobial resistance, and risk factors among slaughterhouse workers, and to identify potentially zoonotic serovars in the pork value chain. Material and methods: We conducted a nationwide cross-sectional survey, collecting 364 stool samples from livestock slaughterhouse workers and 1,535 samples from the pork value chain: mesenteric lymph nodes, fecal samples, swabs of carcass splitting floor, cleaning water, meat handlers hand swabs, carcass swabs, raw pork, cooked pork, and mixed raw vegetables. Samples were cultured for isolation of NTS, and subsequently serotyped according to White–Kauffmann–Le Minor scheme. Antimicrobial resistance profiles were determined using tube microdilution and Sensititre® EUVSEC3® plates. Semi- structured questionnaires with 35 questions were used to collect data on demographics, work related risk factors and activities outside the slaughterhouse. Results and discussion: Overall NTS prevalence was 19.2% (365/1899). Proportions at slaughter were; 46.7% in floor swabs, 30.5% in carcass swabs, 20.5% in pig faeces,19.2% in mesenteric lymph nodes,18.4% in hand swabs, 9.5% in water and 5.2% in slaughterhouse workers. At retail, proportions were 33.8% in pork chopping surface, 33.1% in raw pork, 18.9% in hand swabs, 4.0% in cooked pork and 0.7% in vegetables. Sixty-one serovars were identified, with significant overlap between humans and the pork value chain. Overall, zoonotic S. Zanzibar, monophasic serovars of S. subspecies <i>salamae</i> (II) and subspecies <i>enterica</i> (I), S. Typhimurium and S. Newport, were the most prevalent. S. Typhimurium was predominant in humans and exhibited multi-drug resistance. NTS infection was significantly associated with eating, drinking, or smoking while working (OR = 1.95, 95% CI: 0.67-2.90%, p = 0.004). The detected NTS serovars in slaughterhouse workers could be a potential indicator of circulating serovars in the general population. The persistent presence of NTS along the pork value chain highlights occurrence of cross-contamination and the potential for transmission to consumers and slaughterhouse workers. This emphasizes the need to reduce <i>Salmonella</i> prevalence on pig farms and improve hygiene and pork handling practices at slaughter and retail points.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceAcademicsen_US
dcterms.audienceScientistsen_US
dcterms.available2024-09-17en_US
dcterms.bibliographicCitationKivali, V., Roesel, K., Dohoo, I., Alinaitwe, L., Bugeza, J.K., Hoona, J.J., Mugizi, D.R., Kankya, C., Sinh Dang-Xuan, Szabo, I., Rösler, U., Friese, A. and Cook, E.A.J. 2024. Non-typhoidal <i>Salmonella</i> among slaughterhouse workers and in the pork value chain in selected districts of Uganda. Frontiers in Veterinary Science 11: 1427773.en_US
dcterms.extent1427773en_US
dcterms.issued2024-09-17en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherFrontiers Mediaen_US
dcterms.subjectanimal productsen_US
dcterms.subjectfood safetyen_US
dcterms.subjecthealthen_US
dcterms.subjectswineen_US
dcterms.typeJournal Articleen_US

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