Non-typhoidal Salmonella among slaughterhouse workers and in the pork value chain in selected districts of Uganda

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationFreie Universität Berlinen
cg.contributor.affiliationUniversity of Prince Edward Islanden
cg.contributor.affiliationUniversity of Bernen
cg.contributor.affiliationMinistry of Agriculture, Animal Industry and Fisheries, Ugandaen
cg.contributor.affiliationMakerere Universityen
cg.contributor.affiliationFederal Institute for Risk Assessment, Germanyen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpLivestock
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeOne Health
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierVelma Kivali: 0000-0003-0797-4484
cg.creator.identifierKristina Roesel: 0000-0002-2553-1129
cg.creator.identifierLordrick Alinaitwe: 0000-0002-5432-6407
cg.creator.identifierJames Bugeza: 0000-0002-8124-2807
cg.creator.identifierSinh Dang-Xuan: 0000-0002-0522-7808
cg.creator.identifierElizabeth Cook: 0000-0001-6081-8363
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fvets.2024.1427773en
cg.isijournalISI Journalen
cg.issn2297-1769en
cg.journalFrontiers in Veterinary Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.ilriANIMAL PRODUCTSen
cg.subject.ilriFOOD SAFETYen
cg.subject.ilriHUMAN HEALTHen
cg.subject.ilriPIGSen
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactPlatformNutrition, Health and Food Security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume11en
dc.contributor.authorKivali, Velmaen
dc.contributor.authorRoesel, Kristinaen
dc.contributor.authorDohoo, I.en
dc.contributor.authorAlinaitwe, Lordricken
dc.contributor.authorBugeza, James K.en
dc.contributor.authorHoona, J.J.en
dc.contributor.authorMugizi, Denis R.en
dc.contributor.authorKankya, C.en
dc.contributor.authorSinh Dang-Xuanen
dc.contributor.authorSzabo, I.en
dc.contributor.authorRösler, U.en
dc.contributor.authorFriese, A.en
dc.contributor.authorCook, Elizabeth A.J.en
dc.date.accessioned2024-09-17T16:20:07Zen
dc.date.available2024-09-17T16:20:07Zen
dc.identifier.urihttps://hdl.handle.net/10568/152271
dc.titleNon-typhoidal Salmonella among slaughterhouse workers and in the pork value chain in selected districts of Ugandaen
dcterms.abstractIntroduction: Non-typhoidal <i>Salmonella</i> (NTS) is a major cause of gastroenteritis worldwide, often associated with meat consumption and meat processing. Research on NTS infection and circulating serovars in meat value chains in Uganda is limited. We aimed to establish NTS prevalence, antimicrobial resistance, and risk factors among slaughterhouse workers, and to identify potentially zoonotic serovars in the pork value chain. Material and methods: We conducted a nationwide cross-sectional survey, collecting 364 stool samples from livestock slaughterhouse workers and 1,535 samples from the pork value chain: mesenteric lymph nodes, fecal samples, swabs of carcass splitting floor, cleaning water, meat handlers hand swabs, carcass swabs, raw pork, cooked pork, and mixed raw vegetables. Samples were cultured for isolation of NTS, and subsequently serotyped according to White–Kauffmann–Le Minor scheme. Antimicrobial resistance profiles were determined using tube microdilution and Sensititre® EUVSEC3® plates. Semi- structured questionnaires with 35 questions were used to collect data on demographics, work related risk factors and activities outside the slaughterhouse. Results and discussion: Overall NTS prevalence was 19.2% (365/1899). Proportions at slaughter were; 46.7% in floor swabs, 30.5% in carcass swabs, 20.5% in pig faeces,19.2% in mesenteric lymph nodes,18.4% in hand swabs, 9.5% in water and 5.2% in slaughterhouse workers. At retail, proportions were 33.8% in pork chopping surface, 33.1% in raw pork, 18.9% in hand swabs, 4.0% in cooked pork and 0.7% in vegetables. Sixty-one serovars were identified, with significant overlap between humans and the pork value chain. Overall, zoonotic S. Zanzibar, monophasic serovars of S. subspecies <i>salamae</i> (II) and subspecies <i>enterica</i> (I), S. Typhimurium and S. Newport, were the most prevalent. S. Typhimurium was predominant in humans and exhibited multi-drug resistance. NTS infection was significantly associated with eating, drinking, or smoking while working (OR = 1.95, 95% CI: 0.67-2.90%, p = 0.004). The detected NTS serovars in slaughterhouse workers could be a potential indicator of circulating serovars in the general population. The persistent presence of NTS along the pork value chain highlights occurrence of cross-contamination and the potential for transmission to consumers and slaughterhouse workers. This emphasizes the need to reduce <i>Salmonella</i> prevalence on pig farms and improve hygiene and pork handling practices at slaughter and retail points.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2024-09-17
dcterms.bibliographicCitationKivali, V., Roesel, K., Dohoo, I., Alinaitwe, L., Bugeza, J.K., Hoona, J.J., Mugizi, D.R., Kankya, C., Sinh Dang-Xuan, Szabo, I., Rösler, U., Friese, A. and Cook, E.A.J. 2024. Non-typhoidal <i>Salmonella</i> among slaughterhouse workers and in the pork value chain in selected districts of Uganda. Frontiers in Veterinary Science 11: 1427773.en
dcterms.extent1427773en
dcterms.issued2024-09-17
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectanimal productsen
dcterms.subjectfood safetyen
dcterms.subjecthealthen
dcterms.subjectswineen
dcterms.typeJournal Article

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