Identification of sex-linked markers in the sexually cryptic coco de mer: are males and females produced in equal proportions?

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationUniversity of Exeteren
cg.contributor.affiliationSingapore-ETH Centreen
cg.contributor.affiliationUniversity of Lausanneen
cg.contributor.affiliationSeychelles Islands Foundationen
cg.contributor.affiliationBioversity International and the International Center for Tropical Agricultureen
cg.contributor.affiliationCharles Universityen
cg.contributor.affiliationETH Zürichen
cg.contributor.affiliationBioversity Internationalen
cg.contributor.crpForests, Trees and Agroforestry
cg.contributor.donorDeutsche Forschungsgemeinschaften
cg.contributor.donorRübel Foundationen
cg.coverage.countrySeychelles
cg.coverage.iso3166-alpha2SC
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.coverage.regionSub-Saharan Africa
cg.creator.identifierChris Kettle: 0000-0002-9476-0136en
cg.identifier.doihttps://doi.org/10.1093/aobpla/plz079en
cg.isijournalISI Journalen
cg.issn2041-2851en
cg.issue1en
cg.journalAoB PLANTSen
cg.reviewStatusPeer Reviewen
cg.subject.alliancebiovciatAGRICULTUREen
cg.subject.alliancebiovciatCONSERVATION AND USEen
cg.volume12en
dc.contributor.authorMorgan, Emma J.en
dc.contributor.authorKaiser-Bunbury, Christopheren
dc.contributor.authorEdwards, Peter J.en
dc.contributor.authorScharmann, Mathiasen
dc.contributor.authorWidmer, Alexen
dc.contributor.authorFleischer-Dogley, Fraukeen
dc.contributor.authorKettle, Christopher J.en
dc.date.accessioned2020-04-28T15:32:39Zen
dc.date.available2020-04-28T15:32:39Zen
dc.identifier.urihttps://hdl.handle.net/10568/108086
dc.titleIdentification of sex-linked markers in the sexually cryptic coco de mer: are males and females produced in equal proportions?en
dcterms.abstractLodoicea maldivica (coco de mer) is a long-lived dioecious palm in which male and female plants are visually indistinguishable when immature, only becoming sexually dimorphic as adults, which in natural forest can take as much as 50 years. Most adult populations in the Seychelles exhibit biased sex ratios, but it is unknown whether this is due to different proportions of male and female plants being produced or to differential mortality. In this study, we developed sex-linked markers in Lodoicea using ddRAD sequencing, enabling us to reliably determine the gender of immature individuals. We screened 589 immature individuals to explore sex ratios across life stages in Lodoicea. The two sex-specific markers resulted in the amplification of male-specific bands (Lm123977 at 405 bp and Lm435135 at 130 bp). Our study of four sub-populations of Lodoicea on the islands of Praslin and Curieuse revealed that the two sexes were produced in approximately equal numbers, with no significant deviation from a 1:1 ratio before the adult stage. We conclude that sex in Lodoicea is genetically determined, suggesting that Lodoicea has a chromosomal sex determination system in which males are the heterogametic sex (XY) and females are homogametic (XX). We discuss the potential causes for observed biased sex ratios in adult populations, and the implications of our results for the life history, ecology and conservation management of Lodoicea.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2019-12-18en
dcterms.bibliographicCitationMorgan, E.J.; Kaiser-Bunbury, C.; Edwards, P.J.; Scharmann, M.; Widmer, A.; Fleischer-Dogley, F.; Kettle, C.J. (2020) Identification of sex-linked markers in the sexually cryptic coco de mer: are males and females produced in equal proportions? AoB PLANTS 12(1) 9 p. ISSN: 2041-2851en
dcterms.extent9 p.en
dcterms.issued2020-02-01en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherOxford University Pressen
dcterms.subjectlodoicea maldivicaen
dcterms.subjectconservation agricultureen
dcterms.subjectreproductive behaviouren
dcterms.subjectsex ratioen
dcterms.subjectagricultura de conservaciónen
dcterms.subjectcomportamiento reproductivoen
dcterms.subjectproporción de lo sexosen
dcterms.typeJournal Article

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