Genomic outcomes of haploid induction crosses in potato (Solanum tuberosum L.)

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationUniversity of Californiaen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.crpRoots, Tubers and Bananasen
cg.contributor.donorNational Science Foundation, United Statesen
cg.coverage.countryPeruen
cg.coverage.iso3166-alpha2PEen
cg.coverage.regionLatin Americaen
cg.coverage.regionSouth Americaen
cg.creator.identifierElisa Judith Mihovilovich Castro: 0000-0002-8349-9024en
cg.creator.identifierMerideth Bonierbale: 0000-0002-5157-187Xen
cg.creator.identifierMonica Santayana: 0000-0003-0844-6536en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1534/genetics.119.302843en
cg.isijournalISI Journalen
cg.issn1943-2631en
cg.issue2en
cg.journalGeneticsen
cg.reviewStatusPeer Reviewen
cg.subject.cipBREEDINGen
cg.subject.cipGENETIC RESOURCESen
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCIen
cg.subject.cipPOTATO AGRI-FOOD SYSTEMSen
cg.subject.cipPOTATOESen
cg.subject.cipBIODIVERSITY FOR THE FUTUREen
cg.volume214en
dc.contributor.authorAmundson, K.R.en
dc.contributor.authorOrdoñez, B.en
dc.contributor.authorSantayana, M.en
dc.contributor.authorTan, E.H.en
dc.contributor.authorHenry, I.M.en
dc.contributor.authorMihovilovich, E.en
dc.contributor.authorBonierbale, Merideth W.en
dc.contributor.authorComai, L.en
dc.date.accessioned2020-06-05T00:26:39Zen
dc.date.available2020-06-05T00:26:39Zen
dc.identifier.urihttps://hdl.handle.net/10568/108387
dc.titleGenomic outcomes of haploid induction crosses in potato (Solanum tuberosum L.)en
dcterms.abstractThe challenges of breeding autotetraploid potato (Solanum tuberosum) have motivated the development of alternative breeding strategies. A common approach is to obtain uniparental dihaploids from a tetraploid of interest through pollination with S. tuberosum Andigenum Group (formerly S. phureja) cultivars. The mechanism underlying haploid formation of these crosses is unclear, and questions regarding the frequency of paternal DNA transmission remain. Previous reports have described aneuploid and euploid progeny that, in some cases, displayed genetic markers from the haploid inducer (HI). Here, we surveyed a population of 167 presumed dihaploids for large-scale structural variation that would underlie chromosomal addition from the HI, and for small-scale introgression of genetic markers. In 19 progeny, we detected 10 of the 12 possible trisomies and, in all cases, demonstrated the noninducer parent origin of the additional chromosome. Deep sequencing indicated that occasional, short-tract signals appearing to be of HI origin were better explained as technical artifacts. Leveraging recurring copy number variation patterns, we documented subchromosomal dosage variation indicating segregation of polymorphic maternal haplotypes. Collectively, 52% of the assayed chromosomal loci were classified as dosage variable. Our findings help elucidate the genomic consequences of potato haploid induction and suggest that most potato dihaploids will be free of residual pollinator DNA.en
dcterms.accessRightsLimited Accessen
dcterms.audienceScientistsen
dcterms.audienceCGIARen
dcterms.available2020-02-01en
dcterms.bibliographicCitationAmundson, K.R.; Ordoñez, B.; Santayana, M.; Tan, E.H.; Henry, I.M.; Mihovilovich, E.; Bonierbale, M.W.; Comai, L. 2020. Genomic outcomes of haploid induction crosses in potato (Solanum tuberosum L.). Genetics. ISSN 1943-2631. 214:2. pp. 369-380en
dcterms.extent369-380en
dcterms.issued2020-02-01en
dcterms.languageenen
dcterms.licenseCopyrighted; all rights reserveden
dcterms.publisherOxford University Pressen
dcterms.subjectpotatoesen
dcterms.subjectaneuploidyen
dcterms.subjectgenomicsen
dcterms.subjectsingle nucleotide polymorphismen
dcterms.typeJournal Articleen

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