Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationMaseno Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationSokoine University of Agricultureen
cg.contributor.affiliationUniversity of Embuen
cg.contributor.affiliationUniversity of Liverpoolen
cg.contributor.affiliationCentre Suisse de Recherches Scientifiques en Côte d'Ivoireen
cg.contributor.affiliationNelson Mandela Africa Institution of Science and Technologyen
cg.contributor.affiliationVétérinaires Sans Frontièresen
cg.contributor.affiliationUniversity of Glasgowen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorDELTAS Africa Initiativeen
cg.contributor.donorAfrica Biosciences Challenge Funden
cg.contributor.donorUnited States Defense Threat Reduction Agencyen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierJames Akoko: 0000-0001-5730-4505
cg.creator.identifierRoger Pelle: 0000-0003-1053-085X
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442
cg.creator.identifierEric M. Fèvre: 0000-0001-8931-4986
cg.creator.identifierBernard Bett: 0000-0001-9376-2941
cg.creator.identifierElizabeth Cook: 0000-0001-6081-8363
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1038/s41598-021-88327-zen
cg.isijournalISI Journalen
cg.issn2045-2322en
cg.issue1en
cg.journalScientific Reportsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriBRUCELLOSISen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriZOONOTIC DISEASESen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.volume11en
dc.contributor.authorAkoko, James M.en
dc.contributor.authorPelle, Rogeren
dc.contributor.authorLukambagire, A.S.en
dc.contributor.authorMachuka, Eunice M.en
dc.contributor.authorNthiwa, D.en
dc.contributor.authorMathew, C.en
dc.contributor.authorFèvre, Eric M.en
dc.contributor.authorBett, Bernard K.en
dc.contributor.authorCook, Elizabeth A.J.en
dc.contributor.authorOthero, D.en
dc.contributor.authorBonfoh, Bassirouen
dc.contributor.authorKazwala, R.R.en
dc.contributor.authorShirima, G.en
dc.contributor.authorSchelling, E.en
dc.contributor.authorHalliday, J.E.B.en
dc.contributor.authorOuma, C.en
dc.date.accessioned2021-04-27T12:57:54Zen
dc.date.available2021-04-27T12:57:54Zen
dc.identifier.urihttps://hdl.handle.net/10568/113566
dc.titleMolecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenyaen
dcterms.abstractBrucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.audienceAcademicsen
dcterms.available2021-04-23
dcterms.bibliographicCitationAkoko, J.M., Pelle, R., Lukambagire, A.S., Machuka, E.M., Nthiwa, D., Mathew, C., Fèvre, E.M., Bett, B., Cook, E.A.J., Othero, D., Bonfoh, B., Kazwala, R.R., Shirima, G., Schelling, E., Halliday, J.E.B. and Ouma, C. 2021. Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya. Scientific Reports 11: 8881.en
dcterms.extent8881en
dcterms.issued2021-04-23
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectbrucellaen
dcterms.subjectlivestocken
dcterms.subjectzoonosesen
dcterms.typeJournal Article

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