Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya

Simple item page
cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationMaseno Universityen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationSokoine University of Agricultureen_US
cg.contributor.affiliationUniversity of Embuen_US
cg.contributor.affiliationUniversity of Liverpoolen_US
cg.contributor.affiliationCentre Suisse de Recherches Scientifiques en Côte d'Ivoireen_US
cg.contributor.affiliationNelson Mandela Africa Institution of Science and Technologyen_US
cg.contributor.affiliationVétérinaires Sans Frontièresen_US
cg.contributor.affiliationUniversity of Glasgowen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorDELTAS Africa Initiativeen_US
cg.contributor.donorAfrica Biosciences Challenge Funden_US
cg.contributor.donorUnited States Defense Threat Reduction Agencyen_US
cg.coverage.countryKenyaen_US
cg.coverage.iso3166-alpha2KEen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierJames Akoko: 0000-0001-5730-4505en_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442en_US
cg.creator.identifierEric M. Fèvre: 0000-0001-8931-4986en_US
cg.creator.identifierBernard Bett: 0000-0001-9376-2941en_US
cg.creator.identifierElizabeth Cook: 0000-0001-6081-8363en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1038/s41598-021-88327-zen_US
cg.isijournalISI Journalen_US
cg.issn2045-2322en_US
cg.issue1en_US
cg.journalScientific Reportsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriBRUCELLOSISen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriZOONOTIC DISEASESen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 3 - Good health and well-beingen_US
cg.volume11en_US
dc.contributor.authorAkoko, James M.en_US
dc.contributor.authorPelle, Rogeren_US
dc.contributor.authorLukambagire, A.S.en_US
dc.contributor.authorMachuka, Eunice M.en_US
dc.contributor.authorNthiwa, D.en_US
dc.contributor.authorMathew, C.en_US
dc.contributor.authorFèvre, Eric M.en_US
dc.contributor.authorBett, Bernard K.en_US
dc.contributor.authorCook, Elizabeth A.J.en_US
dc.contributor.authorOthero, D.en_US
dc.contributor.authorBonfoh, Bassirouen_US
dc.contributor.authorKazwala, R.R.en_US
dc.contributor.authorShirima, G.en_US
dc.contributor.authorSchelling, E.en_US
dc.contributor.authorHalliday, J.E.B.en_US
dc.contributor.authorOuma, C.en_US
dc.date.accessioned2021-04-27T12:57:54Zen_US
dc.date.available2021-04-27T12:57:54Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/113566en_US
dc.titleMolecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenyaen_US
dcterms.abstractBrucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.audienceAcademicsen_US
dcterms.available2021-04-23en_US
dcterms.bibliographicCitationAkoko, J.M., Pelle, R., Lukambagire, A.S., Machuka, E.M., Nthiwa, D., Mathew, C., Fèvre, E.M., Bett, B., Cook, E.A.J., Othero, D., Bonfoh, B., Kazwala, R.R., Shirima, G., Schelling, E., Halliday, J.E.B. and Ouma, C. 2021. Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya. Scientific Reports 11: 8881.en_US
dcterms.extent8881en_US
dcterms.issued2021-04-23en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectbrucellaen_US
dcterms.subjectlivestocken_US
dcterms.subjectzoonosesen_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
Co-infection with Rift Valley fever virus, Brucella spp. and Coxiella burnetii in humans and animals in Kenya: Disease burden and ecological factors
CRP A4NH outputs
ILRI animal and human health program outputs (2017-2024)
ILRI BecA articles