Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationLondon School of Hygiene and Tropical Medicineen
cg.contributor.affiliationNational Institute of Veterinary Research, Vietnamen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationNational Institute of Hygiene and Epidemiology, Vietnamen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorAustralian Centre for International Agricultural Researchen
cg.coverage.countryVietnam
cg.coverage.iso3166-alpha2VN
cg.coverage.regionAsia
cg.coverage.regionSouth-eastern Asia
cg.creator.identifierSinh Dang-Xuan: 0000-0002-0522-7808en
cg.creator.identifierHung Nguyen-Viet: 0000-0003-1549-2733en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fvets.2022.816279en
cg.isijournalISI Journalen
cg.issn2297-1769en
cg.journalFrontiers in Veterinary Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.ilriAMRen
cg.subject.ilriANIMAL PRODUCTSen
cg.subject.ilriPIGSen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.volume9en
dc.contributor.authorHolohan, N.en
dc.contributor.authorWallat, M.en
dc.contributor.authorThi Hai Yen Luuen
dc.contributor.authorClark, E.en
dc.contributor.authorDuong Thi Quy Truongen
dc.contributor.authorSinh Dang-Xuanen
dc.contributor.authorHue Thi Kim Vuen
dc.contributor.authorDung Van Truongen
dc.contributor.authorHoang Tran Huyen
dc.contributor.authorHung Nguyen-Vieten
dc.contributor.authorUnger, Freden
dc.contributor.authorSon Thi Thanh Dangen
dc.contributor.authorStabler, R.A.en
dc.date.accessioned2022-04-03T11:21:39Zen
dc.date.available2022-04-03T11:21:39Zen
dc.identifier.urihttps://hdl.handle.net/10568/119225
dc.titleAnalysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencingen
dcterms.abstractNon-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2022-03-29en
dcterms.bibliographicCitationHolohan, N., Wallat, M., Thi Hai Yen Luu, Clark, E., Duong Thi Quy Truong, Sinh Dang-Xuan, Hue Thi Kim Vu, Dung Van Truong, Hoang Tran Huy, Hung Nguyen-Viet, Unger, F., Son Thi Thanh Dang and Stabler, R.A. 2022. Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing. Frontiers in Veterinary Science 9: 816279.en
dcterms.extent816279en
dcterms.issued2022-03-29en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectantimicrobial resistanceen
dcterms.subjectanimal productsen
dcterms.subjectswineen
dcterms.typeJournal Article

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