Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationLondon School of Hygiene and Tropical Medicineen_US
cg.contributor.affiliationNational Institute of Veterinary Research, Vietnamen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationNational Institute of Hygiene and Epidemiology, Vietnamen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorAustralian Centre for International Agricultural Researchen_US
cg.coverage.countryVietnamen_US
cg.coverage.iso3166-alpha2VNen_US
cg.coverage.regionAsiaen_US
cg.coverage.regionSouth-eastern Asiaen_US
cg.creator.identifierSinh Dang-Xuan: 0000-0002-0522-7808en_US
cg.creator.identifierHung Nguyen-Viet: 0000-0003-1549-2733en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.3389/fvets.2022.816279en_US
cg.isijournalISI Journalen_US
cg.issn2297-1769en_US
cg.journalFrontiers in Veterinary Scienceen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.ilriAMRen_US
cg.subject.ilriANIMAL PRODUCTSen_US
cg.subject.ilriPIGSen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 3 - Good health and well-beingen_US
cg.volume9en_US
dc.contributor.authorHolohan, N.en_US
dc.contributor.authorWallat, M.en_US
dc.contributor.authorThi Hai Yen Luuen_US
dc.contributor.authorClark, E.en_US
dc.contributor.authorDuong Thi Quy Truongen_US
dc.contributor.authorSinh Dang-Xuanen_US
dc.contributor.authorHue Thi Kim Vuen_US
dc.contributor.authorDung Van Truongen_US
dc.contributor.authorHoang Tran Huyen_US
dc.contributor.authorHung Nguyen-Vieten_US
dc.contributor.authorUnger, Freden_US
dc.contributor.authorSon Thi Thanh Dangen_US
dc.contributor.authorStabler, R.A.en_US
dc.date.accessioned2022-04-03T11:21:39Zen_US
dc.date.available2022-04-03T11:21:39Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/119225en_US
dc.titleAnalysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencingen_US
dcterms.abstractNon-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceAcademicsen_US
dcterms.audienceScientistsen_US
dcterms.available2022-03-29en_US
dcterms.bibliographicCitationHolohan, N., Wallat, M., Thi Hai Yen Luu, Clark, E., Duong Thi Quy Truong, Sinh Dang-Xuan, Hue Thi Kim Vu, Dung Van Truong, Hoang Tran Huy, Hung Nguyen-Viet, Unger, F., Son Thi Thanh Dang and Stabler, R.A. 2022. Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing. Frontiers in Veterinary Science 9: 816279.en_US
dcterms.extent816279en_US
dcterms.issued2022-03-29en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherFrontiers Mediaen_US
dcterms.subjectantimicrobial resistanceen_US
dcterms.subjectanimal productsen_US
dcterms.subjectswineen_US
dcterms.typeJournal Articleen_US

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Market-based approaches to improving the safety of pork in Vietnam (SafePORK)
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